Expressed sequence tags from cold-acclimatized barley can identify novel plant genes
Expressed sequence tags from cold-acclimatized barley can identify novel plant genes
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DOI：
10.1046/j.1439-0523.2001.00652.x
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发表时间：
2001-12-01
期刊：
影响因子：
2
通讯作者：
Terzi, V
中科院分区：
文献类型：
--
作者：
Faccioli, P;Pecchioni, N;Terzi, V
表达序列标签（est）数据库正在为一些植物物种创建。ESTs具有分析基因组表达部分的潜力，并在从植物生物学理论方面到育种过程的广泛应用中得到应用。本研究基于大麦冷胁迫cDNA文库作为est的来源：对随机分离的克隆进行部分cDNA测序，得到960个est。利用cDNA克隆5′端随机测序策略，可以获得大麦冷胁迫绿叶中转录本稳态水平的信息。重复测序几乎相同的ESTs提供。此外，关于多基因家族存在的有用信息。对这组ESTs进行BLAST检索的结果显示，18.5%的标签与已知基因无显著同源性，10%的标签与已知的冷或其他应激相关基因有显著的同源性，约216%的标签与参与几种代谢途径的酶同源，约15%的标签编码核糖体蛋白、细胞骨架蛋白和历史。泛素，延伸因子等。在某些类别的标签中发现了冗余：RuBisCo， cab蛋白，碳酸酐酶。谷氨酸脱羧酶与应激相关蛋白。
Expressed sequence tags (ESTs) databases are being created for several plant species. ESTs have the potential to analyse the expressed part of the genome, and to be used in a wide range of applications from the theoretical aspect of plant biology to the breeding process. The present study is based on a cold-stressed cDNA library as a source of ESTs in barley: partial cDNA sequencing of randomly isolated clones yielded 960 ESTs. The strategy of random sequencing cDNA clones at their 5' ends allows one to obtain information on the steady-state level of the transcripts in a cold-stressed green leaf of barley. Repetitive sequencing of nearly identical ESTs provides. in addition, useful information on the existence of multigene families. The results of a BLAST search performed on this set of ESTs showed that 18.5% of the tags exhibited no significant homology to known genes, 10% had significant levels of homology with known cold or other stress-related genes, about 216% proved homologous to enzymes involved in several metabolic pathways and about 15% encoded for ribosomal proteins, cytoskeleton proteins, histories. ubiquitin, elongation factors and others. Redundancy was found for some classes of tag: RuBisCo, cab proteins, carbonic anhydrase. glutamate decarboxylase and stress related proteins.