The p75 receptor is required for BDNF-induced differentiation of neural precursor cells
The p75 receptor is required for BDNF-induced differentiation of neural precursor cells
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DOI：
10.1016/s0006-291x(03)00077-9
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发表时间：
2003-02-21
影响因子：
3.1
通讯作者：
Tohyama, M
中科院分区：
文献类型：
--
作者：
Hosomi, S;Yamashita, T;Tohyama, M
表皮生长因子（EGF）处理的胎儿前脑神经球含有多能细胞，能够神经元，星形胶质细胞和少突胶质细胞分化。这些神经前体细胞表达TrkB以及神经营养因子受体p75（p75NTR），表明它们是BDNF响应性的。在这项研究中，我们测试是否p75 NTR在这些神经前体细胞的分化中发挥作用，在体外。通过添加BDNF激活TrkB和p75NTR促进神经元定向和显著的神经突发生。然而，没有促进神经元的承诺，BDNF中观察到的神经前体细胞携带突变的p75 NTR基因的小鼠。此外，我们观察到nestin阳性细胞的数量和缺乏功能性p75 NTR的细胞的增殖显着增加。这些结果表明，p75 NTR是必要的适当的神经元的命运决定，以及神经前体细胞的分化。(C)2003 Elsevier Science（美国）。All rights reserved.
Epidermal growth factor (EGF)-treated neurospheres from fetal forebrain contain multipotential cells capable of neuronal, astrocytic, and oligodendroglial differentiation. These neural precursor cells express the TrkB as well as the neurotrophin receptor p75 (p75NTR), suggesting that they are BDNF responsive. In this study, we test whether the p75NTR plays a role in the differentiation of these neural precursor cells in vitro. Activation of the TrkB and the p75NTR by the addition of BDNF facilitates neuronal commitment and marked neurite genesis. However, no promotion of neuronal commitment by BDNF was observed in the neural precursor cells from mice carrying a mutation in the p75NTR gene. In addition, we observed a significant increase in the number of nestin-positive cells and the proliferation of the cells lacking functional p75NTR. These findings suggest that the p75NTR is required for proper neuronal fate decision as well as the differentiation of the neural precursor cells. (C) 2003 Elsevier Science (USA). All rights reserved.