Dielectrophoretic separation of cancer cells from blood
Dielectrophoretic separation of cancer cells from blood
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DOI：
10.1109/28.585856
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发表时间：
1997-05-01
影响因子：
4.4
通讯作者：
Becker, FF
中科院分区：
文献类型：
--
作者：
Gascoyne, PRC;Wang, XB;Becker, FF
最近的测量表明，细胞的介电特性取决于它们的类型和生理状态。例如，发现MDA-231人乳腺癌细胞的平均质膜比电容为26 mF/m（2），是静息T淋巴细胞所观察到的值（11 mF/m（2））的两倍多。当一个不均匀的交流电场被施加到一个粒子，介电泳（DEP）力的出现取决于粒子的介电性能。因此，具有不同介电特性的细胞在经受这种场时将经历不同的DEP力。在这篇文章中，我们演示了使用不同的DEP力从血液中的介电亲和柱分离几种不同的癌细胞类型。这些分离是使用在底壁上具有微电极阵列的薄的平坦腔室来完成的。通过向电极施加交流场产生的DEP力用于影响细胞通过抛物线流体流动轮廓内的流体动力从室中洗脱的速率。首先对待分离的细胞混合物内发现的各种细胞类型进行电旋转测量，并使用理论建模来导出细胞介电参数。最佳的分离条件，然后预测从频率和悬浮液电导率的依赖性，由这些参数定义的细胞DEP响应。然后在不同浓度下对癌细胞与正常细胞的各种比率进行细胞分离。在分离效率、细胞活力和分离速度方面表征槽纹细胞。例如，在肿瘤与正常细胞比率为1：1 × 10（5）或1：3 × 10（5）时，从血液中清除MDA-231细胞的效率达到100%，细胞活力没有受到损害，并且分离速率为至少10（3）个细胞/秒。这种分离器的设计和操作的理论和实验标准。
Recent measurements have demonstrated that the dielectric properties of cells depend on their type and physiological status. For example, MDA-231 human breast cancer cells were found to have a mean plasma membrane specific capacitance of 26 mF/m(2), more than double the value (11 mF/m(2)) observed for resting T-lymphocytes. When an inhomogeneous ac electric field is applied to a particle, a dielectrophoretic (DEP) force arises that depends on the particle dielectric properties. Therefore, cells having different dielectric characteristics will experience differential DEP forces when subjected to such a field. In this article, we demonstrate the use of differential DEP forces for the separation of several different cancerous cell types from blood in a dielectric affinity column. These separations were accomplished using thin, flat chambers having microelectrode arrays on the bottom wall. DEP forces generated by the application of ac fields to the electrodes were used to influence the rate of elution of cells from the chamber by hydrodynamic forces within a parabolic fluid flow profile. Electrorotation measurements were first made on the various cell types found within cell mixtures to be separated, and theoretical modeling was used to derive the cell dielectric parameters. Optimum separation conditions were then predicted from the frequency and suspension conductivity dependencies of cell DEP responses defined by these parameters. Cell separations were then undertaken for various ratios of cancerous to normal cells at different concentrations. fluted cells were characterized in terms of separation efficiency, cell viability, and separation speed, For example, 100% efficiency was achieved for purging MDA-231 cells from blood at the tumor to normal cell ratio 1:1 x 10(5) or 1:3 x 10(5), cell viability was not compromised, and separation rates were at least 10(3) cells/s. Theoretical and experimental criteria for the design and operation of such separators are presented.