COI and ITS2 sequences delimit species, reveal cryptic taxa and host specificity of fig-associated Sycophila (Hymenoptera, Eurytomidae)
COI and ITS2 sequences delimit species, reveal cryptic taxa and host specificity of fig-associated Sycophila (Hymenoptera, Eurytomidae)
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DOI：
10.1111/j.1755-0998.2009.02671.x
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发表时间：
2010-01-01
影响因子：
7.7
通讯作者：
Huang, Dawei
中科院分区：
文献类型：
--
作者：
Li, Yanwei;Zhou, Xin;Huang, Dawei
虽然属Sycophila具有广泛的主机偏好，一些物种是专门与无花果作为nonpollinator黄蜂。由于它们的两性异形、形态可塑性、隐蔽的交配行为和生物学知之甚少，物种鉴定往往是不确定的。特别难匹配同种的雌性和雄性。本研究利用线粒体COI和核ITS 2两个分子标记对中国6种无花果中的嗜管蚜进行了鉴定。形态学研究揭示了25种雌性和雄性变体，而两种基因的序列结果一致支持15个物种的存在，其中13个是宿主专家，两个使用双宿主。在4种无花果上各发现1种嗜管蚜，而在小叶榕上和小叶榕上各发现6种嗜管蚜。序列分析结果显示，在一个物种中有三种雄性变体，并检测到两个物种被形态学分析所忽略。
Although the genus Sycophila has broad host preferences, some species are specifically associated with figs as nonpollinator wasps. Because of their sexual dimorphism, morphological plasticity, cryptic mating behaviour and poorly known biology, species identifications are often uncertain. It is particularly difficult to match conspecific females and males. In this study, we employed two molecular markers, mitochondrial COI and nuclear ITS2, to identify Sycophila from six Chinese fig species. Morphological studies revealed 25 female and male morphs, while sequence results for both genes were consistent in supporting the presence of 15 species, of which 13 were host specialists and two used dual hosts. A single species of Sycophila was respectively found on four fig species, but six species were isolated from Ficus benjamina and a same number was reared from Ficus microcarpa. Sequence results revealed three male morphs in one species and detected two species that were overlooked by morphological analysis.