Benzo(a)pyrene inhibits migration and invasion of extravillous trophoblast HTR‐8/SVneo cells via activation of the ERK and JNK pathway
Benzo(a)pyrene inhibits migration and invasion of extravillous trophoblast HTR‐8/SVneo cells via activation of the ERK and JNK pathway
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DOI：
10.1002/jat.3227
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发表时间：
2016-07
影响因子：
3.3
通讯作者：
Liyuan Liu;Yingxiong Wang;C. Shen;Junlin He;Xue-qing Liu;Yubin Ding;Rufei Gao;Xuemei Chen
中科院分区：
文献类型：
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作者：
Liyuan Liu;Yingxiong Wang;C. Shen;Junlin He;Xue-qing Liu;Yubin Ding;Rufei Gao;Xuemei Chen
苯并(a)芘（BaP）是一种严重威胁人类健康的持久性有机污染物。大量研究表明，BaP对妊娠有不良影响，但其机制尚不清楚。滋养细胞对子宫内膜的适度浸润是胚胎着床成功的重要因素。本研究旨在探讨BaP对滋养细胞侵袭和迁移的影响及其机制。HTR‐8/SVneo细胞分别用不同浓度（1、5、10、25、50和100 μM）的BaP处理。观察BaP处理后HTR‐8/SVneo细胞的侵袭和迁移。Western blot检测与迁移和侵袭相关的蛋白水平。结果证实，BaP可抑制HTR‐8/SVneo细胞的胞外滋养层迁移和侵袭。进一步的研究表明，BaP处理可以改变HTR‐8/SVneo细胞中MMP‐2、MMP‐9和E‐cadherin的蛋白水平。此外，数据表明BaP激活了MAPK信号通路。使用特异性MAPK抑制剂预处理可以挽救BaP诱导的HTR - 8/SVneo细胞迁移和侵袭的变化。综上所述，我们的研究结果表明，BaP抑制HTR‐8/SVneo细胞的侵袭和迁移，这可能导致妊娠早期失败。版权所有©2015 John Wiley & Sons， Ltd
Benzo(a)pyrene (BaP) is a persistent organic pollutant (POP) that is a serious threat to human health. Numerous studies have shown that BaP causes adverse effects in pregnancy, but the mechanism remains unclear. The moderate invasion of trophoblast cells into the endometrium is an important factor during successful embryo implantation. The aim of this study was to investigate the effect and mechanism of BaP on the invasion and migration of trophoblast cells. HTR‐8/SVneo cells were treated with different concentrations (1, 5, 10, 25, 50 and 100 μM) of BaP. The invasion and migration of HTR‐8/SVneo cells were observed after BaP treatment. The protein levels related to migration and invasion was detected by Western blot. The results confirmed that BaP inhibits the migration and invasion of extravillous trophoblast HTR‐8/SVneo cells. Further investigations indicated that the protein levels of MMP‐2, MMP‐9 and E‐cadherin in HTR‐8/SVneo cells were changed by BaP treatment. Moreover, the data demonstrated that BaP activated the MAPK signaling pathway. Pretreatment with specific inhibitors of MAPK rescued BaP‐induced change in the migration and invasion of HTR‐8/SVneo cells. Taken together, our results indicated that BaP inhibits invasion and the migration of HTR‐8/SVneo cells, which might cause a failure in early pregnancy. Copyright © 2015 John Wiley & Sons, Ltd.