Laboratory evolution of protein conformational dynamics
Laboratory evolution of protein conformational dynamics
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DOI：
10.1016/j.sbi.2017.09.005
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发表时间：
2018-06-01
影响因子：
6.8
通讯作者：
Jackson, Colin J.
中科院分区：
文献类型：
--
作者：
Campbell, Eleanor C.;Correy, Galen J.;Jackson, Colin J.
这篇综述集中在最近的工作，已经开始建立蛋白质构象动力学的特定功能角色，特别是蛋白质可以采样的构象景观如何在基于实验室的进化选择下进化。我们讨论计算和生物物理化学方面的最新技术进展，这为我们提供了剖析进化过程的新方法。最后，我们提供了一些关于构象动力学和演化的新兴观点，以及我们在合理设计构象动力学方面所面临的挑战。
This review focuses on recent work that has begun to establish specific functional roles for protein conformational dynamics, specifically how the conformational landscapes that proteins can sample can evolve under laboratory based evolutionary selection. We discuss recent technical advances in computational and biophysical chemistry, which have provided us with new ways to dissect evolutionary processes. Finally, we offer some perspectives on the emerging view of conformational dynamics and evolution, and the challenges that we face in rationally engineering conformational dynamics.