Mutant GNAS detected in duodenal collections of secretin-stimulated pancreatic juice indicates the presence or emergence of pancreatic cysts
Mutant GNAS detected in duodenal collections of secretin-stimulated pancreatic juice indicates the presence or emergence of pancreatic cysts
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DOI：
10.1136/gutjnl-2012-302823
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发表时间：
2013-07-01
期刊：
影响因子：
24.5
通讯作者：
Goggins, Michael
中科院分区：
文献类型：
--
作者：
Kanda, Mitsuro;Knight, Spencer;Goggins, Michael
目的胰腺囊肿是胰腺影像学检查中常见的疾病。需要更好的方法来治疗这些病变。在这项研究中，我们评估了检测突变体DNA分泌素刺激的胰液中的效用。设计分泌素刺激的胰液收集从十二指肠的291名受试者在胰腺癌筛查试验在五个美国学术医疗中心。研究人群包括接受胰腺筛查的具有胰腺癌家族易感性的受试者，以及正常胰腺、慢性胰腺炎、散发性导管内乳头状粘液性肿瘤（IPMN）或其他肿瘤的疾病对照。结果78例家族性和散发性IPMN中50例（64.1%）、33例（45.5%）仅有小囊肿的患者中15例（45.5%）、15例（45.5%）、15例（45.5%）有分泌素刺激的胰液样本中检测到GNAS突变。
Objective Pancreatic cysts are commonly detected in patients undergoing pancreatic imaging. Better approaches are needed to characterise these lesions. In this study we evaluated the utility of detecting mutant DNA in secretin-stimulated pancreatic juice.Design Secretin-stimulated pancreatic juice was collected from the duodenum of 291 subjects enrolled in Cancer of the Pancreas Screening trials at five US academic medical centres. The study population included subjects with a familial predisposition to pancreatic cancer who underwent pancreatic screening, and disease controls with normal pancreata, chronic pancreatitis, sporadic intraductal papillary mucinous neoplasm (IPMN) or other neoplasms. Somatic GNAS mutations (reported prevalence similar to 66% of IPMNs) were measured using digital high-resolution melt-curve analysis and pyrosequencing.Results GNAS mutations were detected in secretin-stimulated pancreatic juice samples of 50 of 78 familial and sporadic cases of IPMN(s) (64.1%), 15 of 33 (45.5%) with only diminutive cysts (