Intracellular redistribution of nuclear and nucleolar proteins during differentiation of 32D murine hemopoietic cells.
Intracellular redistribution of nuclear and nucleolar proteins during differentiation of 32D murine hemopoietic cells.
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DOI：
10.1016/s0014-4827(03)00178-2
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发表时间：
2003-08
影响因子：
3.7
通讯作者：
X. Tu;R. Baffa;S. Luke;M. Prisco;R. Baserga
中科院分区：
文献类型：
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作者：
X. Tu;R. Baffa;S. Luke;M. Prisco;R. Baserga
我们研究了小鼠造血 32D 和 32D 衍生细胞在指数生长期间和诱导分化后四种蛋白质的细胞内定位。研究的四种蛋白质是胰岛素受体底物-1 (IRS-1)、ID2 蛋白质、核仁素和上游结合因子 (UBF)，所有这些蛋白质都直接或间接参与分化程序。正如预期的那样，这四种蛋白质在指数生长期间主要位于核（和/或核仁）。在沿粒细胞途径诱导分化的三种模型中，IRS-1、ID2和核仁素部分转移到细胞质，在那里它们的水平最终下降。 UBF 在分化过程中也消失，但我们无法检测到该蛋白的细胞质转移。这些实验表明，32D 和 32D 衍生细胞中粒细胞分化的诱导伴随着蛋白质的细胞内重新分布。这种核-细胞质穿梭可能在分化过程中发生的基因表达变化中发挥重要作用。
We have investigated the intracellular localization of four proteins in murine hemopoietic 32D and 32D-derived cells during exponential growth and after induction of differentiation. The four proteins studied were the insulin receptor substrate-1 (IRS-1), the ID2 protein, nucleolin, and the upstream binding factor (UBF), all of which are involved directly or indirectly in the differentiation program. These four proteins were found to be predominantly nuclear (and/or nucleolar) during exponential growth, as expected. In three models of induced differentiation along the granulocytic pathway, IRS-1, ID2, and nucleolin shifted in part to the cytoplasm, where their levels eventually decreased. UBF also disappeared during differentiation, but we could not detect a cytoplasmic shift in this protein. These experiments indicate that induction of granulocytic differentiation in 32D and 32D-derived cells is accompanied by intracellular redistribution of proteins. This nucleo-cytoplasmic shuttle may play a significant role in the changes in gene expression that occur during differentiation.