Evaluation of the epidermal growth factor receptor (EGFR) in colorectal tumours and lymph node metastases
Evaluation of the epidermal growth factor receptor (EGFR) in colorectal tumours and lymph node metastases
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DOI：
10.1016/s0959-8049(02)00234-4
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发表时间：
2002-11-01
影响因子：
8.4
通讯作者：
McLeod, HL
中科院分区：
文献类型：
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作者：
McKay, JA;Murray, LJ;McLeod, HL
表皮生长因子受体(EGFR)的过度表达通常与侵袭性肿瘤表型和预后不良相关。为探讨EGFR与结直肠癌的相关性，我们采用免疫组织化学方法检测了249例结直肠腺癌和42例淋巴结转移组织中EGFR蛋白的表达。此外，我们还研究了114个肿瘤中EGFR基因的(CA)(N)二核苷酸重复多态性。249例标本中有123例(49.4%)表达高水平的EGFR蛋白。结直肠癌中表皮生长因子受体的表达与分化程度有关(P=0.014)。然而，与Dukes的分期、部位、患者年龄或性别无关。EGFR蛋白表达对本组结直肠癌患者的生存无影响(P>0.05)。结直肠癌和淋巴结转移瘤中的表达并不相同，只有17/42(40.5%)的样本显示出相同的EGFR水平(P>；0.05)。(CA)(N)二核苷酸重复等位基因在结直肠腺癌中的分布与EGFR蛋白表达无关(P>；0.05)。这些结果表明，尽管EGFR过度表达是结直肠癌发生中的常见事件，但它并不影响患者的预后。(C)2002爱思唯尔科学有限公司。保留所有权利。
Overexpression of the epidermal growth factor receptor (EGFR) often correlates with an aggressive tumour phenotype and poor prognosis. To examine the relevance of EGFR in colorectal cancer, we determined the expression of EGFR protein in 249 colorectal adenocarcinomas and 42 lymph node metastases using immunohistochemistry. Moreover, we investigated a (CA)(n) dinucleotide repeat polymorphism of the EGFR gene in a subset of 114 tumours. High levels of EGFR protein were observed in 123/249 (49.4%) samples. EGFR expression in colorectal carcinomas correlated with differentiation grade (P = 0.014). However, there were no associations with Dukes' stage, site, patient age or gender. EGFR protein expression did not influence survival in this colorectal cancer patient cohort (P greater than or equal to 0.05). Expression was not identical in paired colorectal tumours and lymph node metastases, with only 17/42 (40.5%) samples showing equivalent EGFR levels (P > 0.05). The distribution of the (CA)(n) dinucleotide repeat alleles in colorectal adenocarcinomas was not associated with EGFR protein expression (P > 0.05). These results indicate that while EGFR overexpression is a common event in colorectal carcinogenesis, it does not influence patient prognosis. (C) 2002 Elsevier Science Ltd. All rights reserved.