Distinct in vivo dynamics of vertebrate SUMO paralogues
Distinct in vivo dynamics of vertebrate SUMO paralogues
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DOI：
10.1091/mbc.e04-07-0589
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发表时间：
2004-12-01
影响因子：
3.3
通讯作者：
Dasso, M
中科院分区：
文献类型：
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作者：
Ayaydin, F;Dasso, M
哺乳动物相扑有三种类似物：相扑-1与相扑-2和相扑-3相似，同源性为45%，而相扑-2和相扑-3彼此同源性为96%。目前尚不清楚相扑-1、-2和-3的功能是独特的、多余的还是相互拮抗的。为了解决这个问题，我们通过使用稳定表达每个哺乳动物相扑蛋白与黄色荧光蛋白(YFP)融合的细胞系来研究单个相扑类似物的动力学。SUMO-2和SUMO-3在整个核质中的分布非常相似，而SUMO-1仅分布在核膜和核仁中。光漂白实验表明，相扑-1的动力学比相扑-2和相扑-3的动力学要慢得多。此外，在不同的亚核结构中，相扑副词的移动性也不同。最后，当细胞退出有丝分裂时，时间和分布在不同的对偶之间是不同的。随着后期核膜的重塑，SUMO-1被募集到核膜上，并迅速积聚到细胞核中。相扑-2和相扑-3较早定位于染色体，末期逐渐积累。综上所述，这些发现表明，哺乳动物相扑-1的利用模式与相扑-2和-3在整个细胞周期中的模式明显不同，认为它在功能上是不同的，并在体内受到特定的调控。
There are three mammalian SUMO paralogues: SUMO-1 is similar to 45% identical to SUMO-2 and SUMO-3, which are 96% identical to each other. It is currently unclear whether SUMO-1, -2, and -3 function in ways that are unique, redundant, or antagonistic. To address this question, we examined the dynamics of individual SUMO paralogues by using cell lines that stably express each of the mammalian SUMO proteins fused to the yellow fluorescent protein (YFP). Whereas SUMO-2 and -3 showed very similar distributions throughout the nucleoplasm, SUMO-1 was uniquely distributed to the nuclear envelope and to the nucleolus. Photobleaching experiments revealed that SUMO-1 dynamics was much slower than SUMO-2 and -3 dynamics. Additionally, the mobility of SUMO paralogues differed between subnuclear structures. Finally, the timing and distributions were dissimilar between paralogues as cells exited from mitosis. SUMO-1 was recruited to nuclear membrane as nuclear envelopes reformed in late anaphase, and accumulated rapidly into the nucleus. SUMO-2 and SUMO-3 localized to chromosome earlier and accumulated gradually during telophase. Together, these findings demonstrate that mammalian SUMO-1 shows patterns of utilization that are clearly discrete from the patterns of SUMO-2 and -3 throughout the cell cycle, arguing that it is functionally distinct and specifically regulated in vivo.