Two-dimensional liquid chromatography study of the human whole saliva proteome
Two-dimensional liquid chromatography study of the human whole saliva proteome
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DOI：
10.1021/pr049911o
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发表时间：
2004-09-01
影响因子：
4.4
通讯作者：
David, LL
中科院分区：
文献类型：
--
作者：
Wilmarth, PA;Riviere, MA;David, LL
采用二维液相色谱法（2-DLC）对人全唾液蛋白质组进行了研究。2-DLC研究能够以高置信度鉴定102种蛋白质，包括大多数已知的唾液蛋白（35）和大量常见的血清蛋白（67）。来自富含脯氨酸的蛋白质的肽，在唾液中丰富，具有不寻常的切割位点，并且通常仅部分胰蛋白酶解。还检测到以前在人类唾液中未观察到的三种蛋白质。与先前报道的二维凝胶电泳研究相比，2-DLC发现了大量的已鉴定蛋白质，包括高分子量，低分子量和富含脯氨酸的蛋白质。
The human whole saliva proteome was investigated using two-dimensional liquid chromatography (2-DLC). The 2-DLC study was able to identify, with high confidence, 102 proteins including most known salivary proteins (35), and a large number of common serum proteins (67). Peptides from proline-rich proteins, abundant in saliva, had unusual cleavage sites and were frequently only partially tryptic. Three proteins not previously observed in human saliva were also detected. Significantly greater numbers of identified proteins, including high molecular weight, low molecular weight, and proline-rich proteins, were found with 2-DLC compared to previously reported two-dimensional gel electrophoresis studies.