Overexpression of Arabidopsis NLP7 improves plant growth under both nitrogen-limiting and -sufficient conditions by enhancing nitrogen and carbon assimilation.
Overexpression of Arabidopsis NLP7 improves plant growth under both nitrogen-limiting and -sufficient conditions by enhancing nitrogen and carbon assimilation.
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拟南芥 NLP7 的过表达通过增强氮和碳同化来改善氮限制和充足条件下的植物生长
DOI：
10.1038/srep27795
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发表时间：
2016-06-13
影响因子：
4.6
通讯作者：
Xiang CB
中科院分区：
文献类型：
--
作者：
Yu LH;Wu J;Tang H;Yuan Y;Wang SM;Wang YP;Zhu QS;Li SG;Xiang CB
氮对于植物的生存和生长至关重要。氮肥的过量施用，造成了严重的环境污染，增加了农业生产成本。为了解决这个问题，世界范围内投入了巨大的努力来提高作物的氮利用能力。然而，迄今为止只取得了有限的成功。在这里，我们报告 NLP7（NIN-LIKE PROTEIN 7）是提高植物氮利用能力的潜在候选者。当在拟南芥中过度表达时，NLP7 在贫氮和富氮条件下都会增加植物生物量，并具有更好发育的根系和降低的芽/根比。 NLP7 过度表达的植物显示出关键氮代谢物、氮吸收、总氮含量以及涉及氮同化和信号传导的基因表达水平显着增加。更重要的是，NLP7的过度表达还增强了光合作用速率和碳同化作用，而NLP7的敲除则损害了氮和碳同化作用。此外，NLP7 还可改善转基因烟草（Nicotiana tabacum）中的植物生长和氮利用。我们的结果表明，NLP7 通过协调增强氮和碳同化，在贫氮和富氮条件下显着改善植物生长，并为作物改良提供线索。
Nitrogen is essential for plant survival and growth. Excessive application of nitrogenous fertilizer has generated serious environment pollution and increased production cost in agriculture. To deal with this problem, tremendous efforts have been invested worldwide to increase the nitrogen use ability of crops. However, only limited success has been achieved to date. Here we report that NLP7 (NIN-LIKE PROTEIN 7) is a potential candidate to improve plant nitrogen use ability. When overexpressed in Arabidopsis, NLP7 increases plant biomass under both nitrogen-poor and -rich conditions with better-developed root system and reduced shoot/root ratio. NLP7–overexpressing plants show a significant increase in key nitrogen metabolites, nitrogen uptake, total nitrogen content, and expression levels of genes involved in nitrogen assimilation and signalling. More importantly, overexpression of NLP7 also enhances photosynthesis rate and carbon assimilation, whereas knockout of NLP7 impaired both nitrogen and carbon assimilation. In addition, NLP7 improves plant growth and nitrogen use in transgenic tobacco (Nicotiana tabacum). Our results demonstrate that NLP7 significantly improves plant growth under both nitrogen-poor and -rich conditions by coordinately enhancing nitrogen and carbon assimilation and sheds light on crop improvement.