Cis-acting effect of the IL1B C-31T polymorphism on IL-1β mRNA expression
Cis-acting effect of the IL1B C-31T polymorphism on IL-1β mRNA expression
复制标题
DOI：
10.1038/sj.gene.6364128
复制
发表时间：
2004-11-01
影响因子：
5
通讯作者：
Ishida, T
中科院分区：
文献类型：
--
作者：
Kimura, R;Nishioka, T;Ishida, T
由于IL-1B基因多态性对IL-1β产生的影响仍存在争议，我们选择了两个基因多态性，通过等位基因特异性转录定量和单倍型分析来检验它们对IL-1αmRNA表达的顺式作用。对于C-31T多态，我们发现-31T等位基因的表达是-31C等位基因的2.2倍。这种较高的转录效率可能与C-31T位于TATA盒中的事实相对应。另一种多态C+3954T不改变转录水平。等位基因特异性转录物定量的使用使我们能够排除多态对基因表达的反式作用，并有助于理解IL1B多态在多因素疾病易感性中的作用。
Since the effect of IL1B polymorphisms on IL-1beta production is still controversial, we selected two polymorphisms to test their cis-acting effect on IL-1alpha mRNA expression by means of the allele-specific transcript quantification and the haplotype analysis. As for the C-31T polymorphism, we found that expression of the -31T allele was 2.2 times of the -31C allele. This higher transcription efficiency may correspond to the fact that C-31T is located in a TATA box. The other polymorphism,C+3954T, did not alter the levels of transcription. The use of the allele-specific transcript quantification enables us to exclude trans-acting effects of polymorphisms on the gene expression and contributes to understanding the roles of the IL1B polymorphisms in susceptibility to multifactorial diseases.