Structural studies on sialylated and sulphated O-glycosidic mannose-linked oligosaccharides in the chondroitin sulphate proteoglycan of brain.
Structural studies on sialylated and sulphated O-glycosidic mannose-linked oligosaccharides in the chondroitin sulphate proteoglycan of brain.
复制标题
脑硫酸软骨素蛋白多糖中唾液酸化和硫酸化O-糖苷甘露糖连接寡糖的结构研究。
DOI：
10.1042/bj2450229
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Margolis,RU
中科院分区：
文献类型：
--
作者：
Krusius,T;Reinhold,VN;Margolis,RK;Margolis,RU
我们之前已经描述了脑硫酸软骨素蛋白聚糖中中性和唾液化的o糖苷甘露糖连接的四糖和硫酸角蛋白多糖链的结构。本论文提供了一系列相关的唾液化和/或硫酸盐化三至五糖通过碱性硼氢化处理的蛋白多糖糖肽释放的信息。采用离子交换色谱法和凝胶过滤法分离所得寡糖，并采用甲基化分析和快速原子轰击质谱法研究其结构性质。通过离子交换层析得到含有[35S]硫酸盐标记的低聚糖的五个组分，每个组分从Sephadex G-50中洗脱为两个分离良好的峰。大分子和小分子分数的表观Mr值随着低聚糖的酸度（和硫酸盐标记）的增加而增加。较大分子大小的部分含有Mr为3000-4500的甘露糖连接的角蛋白硫酸盐短链，以及一些天冬酰胺连接的低聚糖。较小的三到五糖，Mr 800-1400，似乎有一个共同的GlcNac（β 1-3）甘露醇核，并含有一到两个唾液酸和/或硫酸盐残基。
We have previously described the structures of neutral and sialylated O-glycosidic mannose-linked tetrasaccharides and keratan sulphate polysaccharide chains in the chondroitin sulphate proteoglycan of brain. The present paper provides information on a series of related sialylated and/or sulphated tri- to penta-saccharides released by alkaline-borohydride treatment of the proteoglycan glycopeptides. The oligosaccharides were fractionated by ion-exchange chromatography and gel filtration, and their structural properties were studied by methylation analysis and fast-atom-bombardment mass spectrometry. Five fractions containing [35S]sulphate-labelled oligosaccharides were obtained by ion-exchange chromatography, each of which was eluted from Sephadex G-50 as two well-separated peaks. The apparent Mr values of both the large- and small-molecular-size fractions increased with increasing acidity (and sulphate labelling) of the oligosaccharides. The larger-molecular-size fractions contained short mannose-linked keratan sulphate chains of Mr 3000-4500, together with some asparagine-linked oligosaccharides. The smaller tri- to penta-saccharides, of Mr 800-1400, appear to have a common GlcNac(beta 1-3)Manol core, and to contain one to two residues of sialic acid and/or sulphate.