Plant-Derived Polyphenols Interact with Staphylococcal Enterotoxin A and Inhibit Toxin Activity.
Plant-Derived Polyphenols Interact with Staphylococcal Enterotoxin A and Inhibit Toxin Activity.
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DOI：
10.1371/journal.pone.0157082
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Masuda S
中科院分区：
文献类型：
--
作者：
Shimamura Y;Aoki N;Sugiyama Y;Tanaka T;Murata M;Masuda S
研究了16种植物多酚对金黄色葡萄球菌肠毒素A(SEA)毒性的抑制作用。将植物来源的多酚与培养的金黄色葡萄球菌C-29孵育，用Western印迹分析研究这些样品对C-29产生的SEA的影响。12种多酚(0.1~0.5 mg/mL)可抑制抗SEA抗体与SEA的相互作用。我们研究了这些测试样品与SEA孵育后，多酚是否可以直接与SEA相互作用。结果表明，8种多酚(0.25 mg/mL)能显著降低海藻蛋白水平。此外，与SEA相互作用的多酚对SEA诱导的脾细胞增殖的毒素活性也有抑制作用。对SEA毒性有抑制作用的多酚类物质有与SEA相互作用的趋势。特别是含有1或2个六羟基二苯酰基和/或没食子酸基的多酚类化合物，如丁香素、蓖麻黄素、番泻素、长春花素、柯里拉金和香叶素，在低浓度时与SEA作用强烈，并抑制毒素活性。这些多酚可用于预防金黄色葡萄球菌感染和葡萄球菌食物中毒。
This study was performed to investigate the inhibitory effects of 16 different plant-derived polyphenols on the toxicity of staphylococcal enterotoxin A (SEA). Plant-derived polyphenols were incubated with the cultured Staphylococcus aureus C-29 to investigate the effects of these samples on SEA produced from C-29 using Western blot analysis. Twelve polyphenols (0.1–0.5 mg/mL) inhibited the interaction between the anti-SEA antibody and SEA. We examined whether the polyphenols could directly interact with SEA after incubation of these test samples with SEA. As a result, 8 polyphenols (0.25 mg/mL) significantly decreased SEA protein levels. In addition, the polyphenols that interacted with SEA inactivated the toxin activity of splenocyte proliferation induced by SEA. Polyphenols that exerted inhibitory effects on SEA toxic activity had a tendency to interact with SEA. In particular, polyphenol compounds with 1 or 2 hexahydroxydiphenoyl groups and/or a galloyl group, such as eugeniin, castalagin, punicalagin, pedunculagin, corilagin and geraniin, strongly interacted with SEA and inhibited toxin activity at a low concentration. These polyphenols may be used to prevent S. aureus infection and staphylococcal food poisoning.