Resveratrol enhances ultraviolet B-induced cell death through nuclear factor-κB pathway in human epidermoid carcinoma A431 cells
Resveratrol enhances ultraviolet B-induced cell death through nuclear factor-κB pathway in human epidermoid carcinoma A431 cells
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DOI：
10.1016/j.bbrc.2009.04.100
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发表时间：
2009-06-26
影响因子：
3.1
通讯作者：
Shukla, Yogeshwer
中科院分区：
文献类型：
--
作者：
Roy, Preeti;Kalra, Neetu;Shukla, Yogeshwer
白藜芦醇已被报道在几个体内和体外模型中抑制癌症进展，而紫外线B(UVB)是皮肤癌的主要风险，已知可诱导癌细胞中的细胞死亡。在这里，我们研究了白藜芦醇是否能使A431人表皮样癌细胞对UVB诱导的细胞死亡敏感。我们检测了UVB(30mJ/cm(2))和白藜芦醇(60mU/M)对A431细胞的联合作用。UVB辐射或白藜芦醇对A431癌细胞具有抑制增殖和诱导凋亡的作用。然而，白藜芦醇和UVB联合使用与单独使用其中一种药物相比，对A431细胞的增殖抑制作用增强。此外，白藜芦醇和UVB处理A431细胞后，通过阻断丝氨酸536的磷酸化，使核因子-kappaB失活和随后调节Survivin表达的I-kappaBα的降解，从而阻断核因子-kappaB(NF-kappaB)途径。白藜芦醇和UVB处理也降低了重要转录因子信号转导转录激活因子(STAT1)酪氨酸701的磷酸化，进而抑制了磷酸化STAT1向细胞核的转位。此外，白藜芦醇/UVB还可抑制转移蛋白LIMK1，从而降低A431细胞的运动能力。结论：白藜芦醇联合UVB对皮肤癌细胞有协同作用。因此，白藜芦醇是一种潜在的抗皮肤癌的化疗药物。(C)2009 Elsevier Inc.保留所有权利。
Resveratrol has been reported to Suppress cancer progression in several in vivo and in vitro models, whereas ultraviolet B (UVB), a major risk for skin cancer, is known to induce cell death in cancerous cells. Here, we investigated whether resveratrol can sensitize A431 human epidermoid carcinoma cells to UVB-induced cell death. We examined the combined effect of UVB (30 mJ/cm(2)) and resveratrol (60 mu M) on A431 cells. Exposure of A431 carcinoma cells to UVB radiation or resveratrol can inhibit cell proliferation and induce apoptosis. However, the combination of resveratrol and UVB exposure was associated with increased proliferation inhibition of A431 cells compared with either agent alone. Furthermore, results showed that resveratrol and UVB treatment of A431 cells disrupted the nuclear factor-kappaB (NF-kappa B) pathway by blocking phosphorylation of serine 536 and inactivating NF-kappa B and subsequent degradation of I kappa B alpha, which regulates the expression Of survivin. Resveratrol and UVB treatment also decreased the phosphorylation of tyrosine 701 of the important transcription factor signal transducer activator of transcription (STAT1), which in turn inhibited translocation of phospho-STAT1 to the nucleus. Moreover, resveratrol/UVB also inhibited the metastatic protein LIMK1, which reduced the motility of A431 cells. It) conclusion, our study demonstrates that the combination Of resveratrol and UVB act synergistically against skin cancer cells. Thus, resveratrol is a potential chemotherapeutic agent against skin carcinogenesis. (C) 2009 Elsevier Inc. All rights reserved.