Anionic polythiophene derivative as peroxidase mimetics and their application for detection of hydrogen peroxide and glucose.
Anionic polythiophene derivative as peroxidase mimetics and their application for detection of hydrogen peroxide and glucose.
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DOI：
10.1016/j.talanta.2013.06.063
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发表时间：
2013-10
期刊：
影响因子：
6.1
通讯作者：
Mei Liu;Baoxin Li;Xiang Cui
中科院分区：
文献类型：
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作者：
Mei Liu;Baoxin Li;Xiang Cui
本文研究了阴离子聚噻吩衍生物聚[2-（3-噻吩基）乙氧基-4-丁基磺酸盐]（PTEBS）在H2O2存在下催化过氧化物酶底物3，3 ′，5，5 ′-四甲基联苯胺（TMB）反应产生蓝色显色反应，从而实现H2O2的比色检测。PTEBS具有催化效率高、稳定性好、反应速度快等优点。通过偶联葡萄糖氧化酶催化葡萄糖氧化反应，建立了一种简单、廉价、高灵敏度和高选择性的比色法测定葡萄糖。在0.01 - 0.5 mM范围内，吸光度与葡萄糖浓度成正比，检测限为0.004 mM。这一工作不仅对更好地理解聚噻吩衍生物的独特性质具有重要意义，而且在医学诊断和生物技术方面也具有巨大的潜力。
In this paper, we discovered that the anionic polythiophenes derivative, poly[2-(3-thienyl)ethyloxy-4-butylsulfonate] (PTEBS), possesses intrinsic peroxidase-like activity that can catalyze the reaction of peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2to produce a blue color reaction, which provides colorimetric detection of H2O2. PTEBS exhibits several advantages such as high catalytic efficiency, good stability, and rapid response over horseradish peroxidase (HRP). By coupling the oxidation of glucose catalyzed by glucose oxidase, a simple, inexpensive, highly sensitive and selective colorimetric method for glucose detection has been developed. The absorbance was proportional to the concentration of glucose in the range from 0.01 to 0.5 mM with a detection limit of 0.004 mM. This work is not only of importance for a better understanding of the unique properties of polythiophenes derivative but also of great potential for medical diagnostics and biotechnology.