Perturbation of nuclear architecture by long-distance chromosome interactions
Perturbation of nuclear architecture by long-distance chromosome interactions
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DOI：
10.1016/s0092-8674(00)81240-4
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发表时间：
1996-05-31
期刊：
影响因子：
64.5
通讯作者：
Sedat, JW
中科院分区：
文献类型：
--
作者：
Dernburg, AF;Broman, KW;Sedat, JW
位置效应花斑（PEV）描述了位于异染色质附近的基因的随机转录沉默。我们利用荧光原位杂交（FISH）技术检测了果蝇中眼色基因brown的花斑表达是否受其核定位的影响。在胚胎细胞核中，brown基因座处的异染色质插入总是在空间上与其他异染色质相隔离。然而，在幼虫发育过程中，这种插入会以随机的方式与同一染色体上的其他异染色质区域发生物理关联。这些观察结果表明，brown基因因与着丝粒异染色质的特定接触而沉默。此外，它们为长距离染色体相互作用及其对三维核结构的影响提供了直接证据，同时为位置效应花斑现象提供了一个连贯的解释。
Position-effect variegation (PEV) describes the stochastic transcriptional silencing of a gene positioned adjacent to heterochromatin. Using FISH, we have tested whether variegated expression of the eye-color gene brown in Drosophila is influenced by its nuclear localization. In embryonic nuclei, a heterochromatic insertion at the brown locus is always spatially isolated from other heterochromatin. However, during larval development this insertion physically associates with other heterochromatic regions on the same chromosome in a stochastic manner. These observations indicate that the brown gene is silenced by specific contact with centromeric heterochromatin. Moreover, they provide direct evidence for long-range chromosome interactions and their impact on three-dimensional nuclear architecture, while providing a cohesive explanation for the phenomenon of PEV.