Type III machines of pathogenic yersiniae secrete virulence factors into the extracellular milieu
Type III machines of pathogenic yersiniae secrete virulence factors into the extracellular milieu
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DOI：
10.1046/j.1365-2958.1999.01270.x
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发表时间：
1999-03-01
影响因子：
3.6
通讯作者：
Schneewind, O
中科院分区：
文献类型：
--
作者：
Lee, VT;Schneewind, O
革兰氏阴性细菌使用III型机器将有毒蛋白质注入真核细胞的胞浆中。致病性耶尔森氏菌通过III型途径输出14种YOP蛋白，其中一些被称为效应器YOP，靶向巨噬细胞，从而防止吞噬并允许细菌在淋巴组织内复制。到目前为止，YopB/YopD被认为插入巨噬细胞的质膜，并促进效应YOPs进入真核细胞胞浆。我们在这里表明，耶尔森亚科的III型机器向细胞外环境分泌三种蛋白质(YopB、YopD和YopR)。虽然细菌内的YopD是将毒素注入真核细胞所必需的，但分泌型YopB、YopD和YopR在这一过程中是必不可少的。然而，YopB、YopD和YopR对于在小鼠模型系统中建立耶尔森氏菌感染是必不可少的，这表明III型分泌机也具有将毒力因子输送到细胞外环境的功能。
Gram-negative bacteria use type III machines to inject toxic proteins into the cytosol of eukaryotic cells. Pathogenic Yersinia species export 14 Yop proteins by the type III pathway and some of these, named effector Yops, are targeted into macrophages, thereby preventing phagocytosis and allowing bacterial replication within lymphoid tissues. Hitherto, YopB/YopD were thought to insert into the plasma membrane of macrophages and to promote the import of effector Yops into the eukaryotic cytosol. We show here that the type III machines of yersiniae secrete three proteins into the extracellular milieu (YopB, YopD and YopR). Although intrabacterial YopD is required for the injection of toxins into eukaryotic cells, secreted YopB, YopD and YopR are dispensable for this process. Nevertheless, YopB, YopD and YopR are essential for the establishment of Yersinia infections in a mouse model system, suggesting that type III secretion machines function to deliver virulence factors into the extracellular milieu also.