Germinal center founder cells display propensity for apoptosis before onset of somatic mutation.
Germinal center founder cells display propensity for apoptosis before onset of somatic mutation.
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DOI：
10.1084/jem.185.3.563
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发表时间：
1997-02-03
影响因子：
15.3
通讯作者：
Liu, Y J
中科院分区：
文献类型：
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作者：
Lebecque, S;de Bouteiller, O;Arpin, C;Banchereau, J;Liu, Y J
B淋巴细胞在生发中心内经历其抗原受体的亲和力成熟。这些解剖结构在次级淋巴器官中从少数抗原特异性创始者B细胞的克隆扩增中发展，其分离和表征在此报道。人生殖中心创始细胞表达幼稚B细胞标志物表面IgM和IgD以及生殖中心B细胞标志物CD 10和CD 38。它们表达低水平的Bcl-2，高水平的Fas，并在培养中经历快速凋亡。较小的非增殖sIgM+IgD+ CD 38 + B细胞显示较低水平的体细胞突变，在其免疫球蛋白可变区基因相比，大的增殖。因此，未突变的sIgM+IgD+ CD 38+扁桃体B细胞可能代表生殖中心创始细胞，其中凋亡细胞死亡的程序在体细胞突变开始之前被触发，从而允许在早期阶段选择生殖系抗体库。
B lymphocytes undergo affinity maturation of their antigen receptors within germinal centers. These anatomical structures develop in secondary lymphoid organs from the clonal expansion of a few antigen-specific founder B cells, whose isolation and characterization are reported here. Human germinal center founder cells express the naive B cell markers surface IgM and IgD as well as the germinal center B cell markers CD10 and CD38. They express low levels of Bcl-2, high levels of Fas, and undergo rapid apoptosis in culture. The smaller nonproliferating sIgM+IgD+CD38+ B cells displayed a lower level of somatic mutation in their immunoglobulin variable region genes compared with the large proliferating ones. Unmutated sIgM+IgD+CD38+ tonsillar B cells may thus represent germinal center founder cells in which the program for apoptotic cell death is triggered before the onset of somatic mutation, allowing the selection of the germline antibody repertoire at an early stage.