N-MYC AND C-MYC ONCOGENES AMPLIFICATION IN MEDULLOBLASTOMAS - EVIDENCE OF PARTICULARLY AGGRESSIVE-BEHAVIOR OF A TUMOR WITH C-MYC AMPLIFICATION
N-MYC AND C-MYC ONCOGENES AMPLIFICATION IN MEDULLOBLASTOMAS - EVIDENCE OF PARTICULARLY AGGRESSIVE-BEHAVIOR OF A TUMOR WITH C-MYC AMPLIFICATION
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DOI：
10.1177/030089169107700205
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发表时间：
1991-04-30
期刊：
影响因子：
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通讯作者：
GIANGASPERO, F
中科院分区：
文献类型：
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作者：
BADIALI, M;PESSION, A;GIANGASPERO, F
在 27 个髓母细胞瘤中研究了 N-myc 和 c-myc 扩增。从 19 个福尔马林固定和石蜡包埋的肿瘤以及另外 8 个病例的新鲜冷冻肿瘤组织中提取 DNA。结果显示，没有N-myc癌基因扩增的证据，只有1例c-myc癌基因扩增了27倍。从细胞遗传学角度来看，这种肿瘤呈现出许多双微小染色体（DM）。此外，它具有不寻常的快速侵袭性病程，伴有大量脑脊液播散，对鞘内化疗无反应。我们的结果表明，髓母细胞瘤中 N-myc 和 c-myc 基因扩增的发生率较低，表明这些肿瘤的致癌机制与 DNA 基因扩增并不密切相关。 C-myc 扩增虽然不经常观察到，但可能为髓母细胞瘤细胞在体内提供生长优势，有利于它们的快速传播。具有 c-myc 激活的髓母细胞瘤可能代表具有更具侵袭性行为的肿瘤亚组。
N-myc and c-myc amplification was investigated in 27 medulloblastomas. DNA was extracted from 19 formalin fixed and paraffin embedded tumors and from fresh frozen tumor tissue in 8 other cases. The results showed no evidence of amplification of N-myc oncogene and only 1 case had a 27 fold amplification of c-myc. Cytogenetically, this neoplasm presented numerous double minute chromosomes (DMs). Moreover, it had an unusual rapidly aggressive course with massive cerebrospinal fluid dissemination unresponsive to intrathecal chemotherapy. Our results indicate a low incidence of N-myc and c-myc gene amplification in medulloblastomas, suggesting that the oncogenic mechanism in these neoplasms is not closely related to DNA gene amplification. C-myc amplification, although not frequently observed, may however provide a growth advantage for medulloblastoma cells in vivo, favoring their rapid dissemination. Medulloblastomas with c-myc activation may represent a subgroup of tumors with a more aggressive behavior.