Myo-Inositol content determined by myo-inositol biosynthesis and oxidation in blueberry fruit
Myo-Inositol content determined by myo-inositol biosynthesis and oxidation in blueberry fruit
复制标题
蓝莓果实中肌醇生物合成和氧化测定肌醇含量
DOI：
10.1016/j.foodchem.2016.04.099
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发表时间：
2016-11-01
期刊：
影响因子：
8.8
通讯作者：
Cheng, Xianhao
中科院分区：
文献类型：
--
作者：
Song, Fangyuan;Su, Hongyan;Cheng, Xianhao
肌醇在植物可食器官中的代谢因其对人体健康的益处和在植物发育中的独特功能而成为近年来研究的热点。在这项研究中，肌醇含量的两个蓝莓品种，伯克利和蓝果品种的发展过程中进行了分析。此外，还首次从蓝莓中分离到2个VcMIPS 1/2（Vaccinium corymbosum MIPS）基因、1个VcIMP（Vaccinium corymbosum IMP）基因和1个VcMIOX（Vaccinium corymbosum MIOX）基因。VcMIPS 2、VcIMP和VcMIOX基因的表达模式与果实成熟过程中肌醇含量的变化有一定的关系。酶活分析进一步证实了上述结果。结果表明，在蓝莓生长发育过程中，肌醇的生物合成和氧化对肌醇含量的决定起着重要作用。据我们所知，这份报告是第一次讨论肌醇在水果中的生物合成和催化水平。(C)2016爱思唯尔有限公司版权所有
Myo-inositol metabolism in plant edible organs has become the focus of many recent studies because of its benefits to human health and unique functions in plant development. In this study, myo-inositol contents were analyzed during the development of two blueberry cultivars, cv 'Berkeley' and cv 'Bluecrop'. Furthermore, two VcMIPS 1/2 (Vaccinium corymbosum MIPS) genes, one VcIMP (Vaccinium corymbosum IMP) gene and one VcMIOX (Vaccinium corymbosum MIOX) gene were isolated for the first time from blueberry. The expression patterns of VcMIPS2, VcIMP and VcMIOX genes showed a relationship with the change profiles of myo-inositol content during fruit ripening. The results were further confirmed by the analyses of the enzyme activity. Results indicated that both myo-inositol biosynthesis and oxidation played important roles in determining of myo-inositol levels during the development of blueberry. To our knowledge, this report is the first to discuss myo-inositol levels in fruits in terms of biosynthesis and catabolism. (C) 2016 Elsevier Ltd. All rights reserved.