DNA immunization: effect of secretion of DNA-expressed hemagglutinins on antibody responses.
DNA immunization: effect of secretion of DNA-expressed hemagglutinins on antibody responses.
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DNA 免疫：DNA 表达的血凝素的分泌对抗体反应的影响。
DOI：
10.1016/s0264-410x(99)00345-x
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发表时间：
1999
期刊：
影响因子：
5.5
通讯作者：
Robinson,HL
中科院分区：
文献类型：
--
作者：
Torres,CA;Yang,K;Mustafa,F;Robinson,HL
表达质膜和分泌形式的流感和麻疹病毒血凝素（HA）的DNA疫苗已被用于评估分泌对DNA引起的抗体应答的影响。在低剂量的DNA下，流感病毒HA的质膜形式比分泌形式产生更高的抗体滴度。DNA产生的抗体的同种型取决于DNA递送方法和表达抗原的形式。肌内注射后，表达流感和麻疹HA的膜结合形式的DNA主要产生IgG 2a。相比之下，表达两种HA的分泌形式以及另一种分泌蛋白（人生长激素）的DNA主要产生IgG 1。基因枪递送导致分泌型和膜结合型血凝素的主要IgG 1抗体应答。通过i.m.分泌形式的流感血凝素的递送是IL-4依赖性的，这表明已经产生了T辅助细胞2偏向的免疫应答。
DNA vaccines expressing plasma membrane and secreted forms of the influenza and measles virus hemagglutinins (HAs) have been used to evaluate the effect of secretion on DNA-raised antibody responses. At low doses of DNA, the plasma membrane form of the influenza virus HA raised higher titers of antibody than the secreted form. The isotype of the DNA-raised antibodies depended on both the method of DNA delivery and the form of the expressed antigen. Following intramuscular injections, DNAs expressing membrane bound forms of the influenza and measles HAs raised predominantly IgG2a. By contrast, DNAs expressing the secreted from of the two HAs as well as another secreted protein, human growth hormone, raised predominantly IgG1. Gene gun delivery resulted in predominantly IgG1 antibody responses for both secreted and membrane bound forms of the hemagglutinins. The raising of predominantly IgG1 by i.m. delivery of the secreted form of the influenza hemagglutinin was IL-4 dependent suggesting that a T-helper 2-biased immune response had been raised.