Tracking whisker and head movements in unrestrained behaving rodents

Tracking whisker and head movements in unrestrained behaving rodents
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DOI:
10.1152/jn.00718.2004
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发表时间:
2005-04-01
影响因子:
2.5
通讯作者:
Ahissar, E
Ahissar, E
中科院分区:
医学3区
文献类型:
--
作者:
Knutsen, PM;Derdikman, D;Ahissar, E

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由于最近的进展使得能够在清醒行为的啮齿类动物的大脑中进行实时电生理记录,因此需要有效的方法来以毫秒分辨率分析由此产生的大量行为数据。我们描述了一种半自动、有效的方法,可以使用高速视频准确跟踪自由移动的啮齿动物的头部和神秘触须(胡须)运动。通过跟踪单个胡须的整个长度,我们展示了在描述胡须相关任务和探索行为期间胡须运动的运动学以及胡须与物体的相互作用时,胡须的位置和形状如何相关。
Due to recent advances that enable real-time electrophysiological recordings in brains of awake behaving rodents, effective methods for analyzing the large amount of behavioral data thus generated, at millisecond resolution, are required. We describe a semiautomated, efficient method for accurate tracking of head and mystacial vibrissae (whisker) movements in freely moving rodents using high-speed video. By tracking the entire length of individual whiskers, we show how both location and shape of whiskers are relevant when describing the kinematics of whisker movements and whisker interactions with objects during a whisker-dependent task and exploratory behavior.