Detection of RAPD markers linked to the everbearing gene in Japanese cultivated strawberry
Detection of RAPD markers linked to the everbearing gene in Japanese cultivated strawberry
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DOI：
10.1111/j.1439-0523.2005.01144.x
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发表时间：
2005-10-01
期刊：
影响因子：
2
通讯作者：
Watanabe, K
中科院分区：
文献类型：
--
作者：
Sugimoto, T;Tamaki, K;Watanabe, K
为了鉴定草莓中常生基因的标记，从“Ever Berry”（日本常生草莓）和“Toyonoka”（日本六月草莓）杂交产生 199 个 F-1 后代植物作为实验群体。开花测试结果产生了 97 株常熊和 102 株六月熊。卡方检验给出了 Everbears 与 Junebears 的预期比例 1:1 的拟合优度，表明 Everbears 性状的遗传是由单基因显性基因控制的。使用‘Ever Berry’和‘Toyonka’对该性状进行了 RAPD 分析。从总共 175 个引物中鉴定出 71 个引物，在两个亲本之间产生 89 个多态性片段。从 199 株后代植物中的 26 株中选出了 5 个与常生性状相关的标记。用这五个标记分析剩余的 173 株幼苗，并使用所有 199 株 F-1 后代植物构建连锁图谱。该连锁群的长度为 39.7 cM。发现的最接近的标记 OPE07-1 和 OPB05-1 分别定位在常生基因两侧的 11.8 和 15.8 cM 处。
To identify markers for the everbearing gene in strawberries, 199 F-1 progeny plants were produced from a cross between 'Ever Berry' (a Japanese everbearing strawberry) and 'Toyonoka' (a Japanese Junebearing strawberry) as the experimental population. The results of flowering tests produced 97 everbears and 102 Junebears. The chi-square test gave a goodness of fit for the expected ratio of 1 : 1 for everbears to Junebears, suggesting the inheritance of the everbearing trait is controlled by a monogenic dominant gene. RAPD analyses on this trait were carried out using 'Ever Berry' and 'Toyonoka'. Seventy-one primers, which produced 89 polymorphic fragments between the two parents, were identified from a total of 175 primers. Five markers relating to the everbearing trait were selected from 26 of the 199 progeny plants. The remaining 173 seedlings were analysed with these five markers and a linkage map was constructed using all of the 199 F-1 progeny plants. The length of this linkage group is 39.7 cM. The closest markers found, OPE07-1 and OPB05-1, are respectively mapped at 11.8 and 15.8 cM on each side of the everbearing gene.