Evaluation of kudzu root extract-induced hepatotoxicity
Evaluation of kudzu root extract-induced hepatotoxicity
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葛根提取物诱导的肝毒性评价
DOI：
10.1016/j.jep.2015.11.005
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发表时间：
2015-12-24
影响因子：
5.4
通讯作者：
Xu, Feng
中科院分区：
文献类型：
--
作者：
Wang, Dengyuan;Qiu, Liang;Xu, Feng
民族药理学相关性：葛根，野葛根。几个世纪以来，葛根一直被用作食物和药物，但很少有研究表明葛根可能会导致肝脏损伤。研究目的：研究葛根提取物对小鼠、HepG2细胞和小鼠肝细胞的肝毒性。材料与方法：小鼠灌胃葛根提取物（10 mg/d） 4周，进行生化分析和组织病理学改变。为探讨葛根提取物诱导小鼠肝毒性的可能机制，将小鼠肝细胞HepG2细胞与葛根提取物或葛根素（葛根异黄酮的一种）共培养2 h。结果：小鼠血清ALT和AST升高，葛根提取物具有肝毒性。对HepG2细胞和小鼠肝细胞LDH渗漏的增加进一步证实了葛根提取物的肝毒性。葛根提取物和葛根素显著上调参与急性期反应和Box的Mt1 mRNA，这对细胞凋亡至关重要。治疗组Gclc、Nrf2、Ho-1 mRNA表达无明显变化。结论：葛根提取物可能具有肝毒性，应谨慎使用。2015爱思唯尔爱尔兰有限公司版权所有。
Ethnopharmacological relevance: Kudzu root, the root of Pueraria lobata (Willd.) Ohwi, has been used as food and medicine for centuries, but few studies indicate that kudzu root may cause liver damage.Aim of study: We studied the hepatotoxicity of kudzu root extract in mice, HepG2 cells and mice hepatocytes.Materials and methods: Mice were administrated with kudzu root extract (10 mg/day) for 4 weeks, and then the biochemical analysis and histopathological changes were carried out. To explore the potential mechanism by which kudzu root extract-induced hepatotoxicity, HepG2 cells and mice hepatocytes were co-cultured with kudzu root extract or puerarin, which is a kudzu root isoflavone, for 2 h.Results: The increase of serum ALT and AST and histopathological changes in treated mice revealed that kudzu root extract was hepatotoxic. The increase of LDH leakage for HepG2 cells and mice hepatocytes further confirmed hepatotoxicity of kudzu root extract. Kudzu root extract and puerarin significantly up-regulated Mt1 mRNA involved in the acute phase response and Box which is crucial for apoptosis. Gclc, Nrf2 and Ho-1 mRNA expressions did not change in treatment group.Conclusions: Kudzu root extract may be hepatotoxic and caution may be required for its use. (c) 2015 Elsevier Ireland Ltd. All rights reserved.