Transfer of antibiotic multiresistant plasmid RP4 from escherichia coli to activated sludge bacteria.
Transfer of antibiotic multiresistant plasmid RP4 from escherichia coli to activated sludge bacteria.
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DOI：
10.1263/jbb.106.292
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发表时间：
2008-09
影响因子：
2.8
通讯作者：
S. Soda;Hidetaka Otsuki;D. Inoue;H. Tsutsui;K. Sei;M. Ike
中科院分区：
文献类型：
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作者：
S. Soda;Hidetaka Otsuki;D. Inoue;H. Tsutsui;K. Sei;M. Ike
使用过滤器交配研究了自传播、抗生素多重耐药性质粒 RP4 从实验室大肠杆菌菌株 C600 到本地活性污泥细菌的原位转移。 RP4从供体大肠杆菌到从两个废水处理厂取样的细菌的转移频率为5.1x10(-2)至7.5x10(-1)和4.6x10(-3)至1.3x10(-2)/潜在受体。分离的转接合子表现出对 Ap、Km 和 Tc 的抗性，并且存在与 RP4 大小相似的质粒。 RP4 上的 traG 基因也在所有转接合子中检测到。从转接合子到大肠杆菌HB101的反向转移实验表明，RP4在转接合子中保持了自我传播性。转接合菌株是活性污泥中的优势细菌，包括荧光假单胞菌、恶臭假单胞菌和人苍白杆菌，以及少数肠道细菌菌株，包括弗氏柠檬酸杆菌、大肠杆菌、阴沟肠杆菌、阿氏肠杆菌和肺炎克雷伯菌。肺炎杆菌。转接合菌株肺炎克雷伯菌亚种。肺炎球菌、阴沟肠球菌和阿斯布里亚肠球菌有几种天然存在的质粒。这些结果表明，活性污泥中的释放细菌和本地细菌之间可能发生质粒的原位转移和抗生素抗性基因的交换。
In situ transfer of a self-transmissible, antibiotic-multiresistant plasmid RP4 from a laboratory Escherichia coli strain C600 to indigenous activated sludge bacteria was investigated using filter mating. The transfer frequency of RP4 from the donor E. coli to the bacteria that was sampled from two wastewater treatment plants was 5.1x10(-2) to 7.5x10(-1) and 4.6x10(-3) to 1.3x10(-2)/potential recipient. The isolated transconjugants showed resistance to Ap, Km, and Tc and the presence of a plasmid with a similar size to RP4. The traG gene on RP4 was also detected from all transconjugants. Reverse-transfer experiments from the transconjugants to E. coli HB101 indicated that RP4 maintained self-transmissibility in the transconjugants. The transconjugant strains were dominant bacteria in activated sludge including Pseudomonas fluorescens, P. putida, and Ochrobactrum anthropi and minor populations of enteric bacterial strains including Citrobacter freundii, E. coli, Enterobacter cloacae, E. asburiae, and Klebsiella pneumoniae ssp. pneumoniae. The transconjugant strains K. pneumoniae ssp. pneumonia, E. cloacae, and E. asburiae had several naturally occurring plasmids. These results suggest that in situ transfer of plasmids and the exchange of antibiotic-resistant genes can occur between released and indigenous bacteria in activated sludge.