A new approach for standard preparation in microbeam analysis: Development and validation
A new approach for standard preparation in microbeam analysis: Development and validation
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DOI：
10.1007/s10967-008-7312-6
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发表时间：
2009-02-01
影响因子：
1.6
通讯作者：
Yukawa, M.
中科院分区：
文献类型：
--
作者：
Homma-Takeda, S.;Nishimura, Y.;Yukawa, M.
微束分析用于生物医学和环境科学，以确定微量元素的存在和浓度。然而，生物样品的定量分析具有挑战性，因为现有标准的化学和物理成分与生物样品中通常遇到的化学和物理成分不同。我们使用聚乙烯醇开发了一种薄标准品，并通过微束扫描粒子诱导 X 射线发射 (micro-PIXE) 分析评估其质量。对于高达 500 μg 中心点 g(-1) 的某些标准，金属浓度和 X 射线强度之间的关系呈线性。使用这种新的薄标准品，对人类头发样品中的锌含量进行的微 PIXE 分析与电感耦合等离子体质谱法进行的分析非常吻合，验证了这些新的薄标准品在微束分析定量绘图中的使用。
Microbeam analysis is used in biomedical and environmental sciences to determine the presence and concentration of trace elements. However, quantitative analyses of biological samples are challenging because the chemical and physical compositions of existing standards differ from those typically encountered in biological samples. We developed a thin standard using polyvinyl alcohol and assessed its quality by microbeam scanning particle induced X-ray emission (micro-PIXE) analysis. The relationship between metal concentration and X-ray intensity was linear for certain standards up to 500 mu g center dot g(-1). Using this new thin standard, micro-PIXE analysis of Zn content in samples of human hair agreed well with analysis performed by inductively-coupled plasma mass spectrometry, validating the use of these new thin standards for quantitative mapping with microbeam analysis.