Vascular tone and neurovascular coupling: considerations toward an improved in vitro model.
Vascular tone and neurovascular coupling: considerations toward an improved in vitro model.
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DOI：
10.3389/fnene.2010.00016
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Filosa, Jessica A
中科院分区：
文献类型：
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作者：
Filosa, Jessica A
神经血管研究在理解脑神经血管单位如何在神经元激活后实现血流的快速和空间增加方面取得了重大进展。在使用的实验模型中，体外脑切片制备提供了独特的信息，揭示了与功能性充血有关的潜在信号和细胞机制。然而，该模型最关键的限制是缺乏所研究血管的腔内压力和流量。此外，基础血管张力的差异导致了对神经元到神经胶质刺激后血管反应极性的不同解释。鉴于星形胶质细胞诱导的神经血管反应的复杂性，我们建议使用改良的体外脑切片制备，其中保留腔内小动脉压力和血流。在这篇综述中，我们讨论了当使用脑切片进行神经血管研究时需要考虑的优点和缺点。提出了克服当前限制的潜在方法。
Neurovascular research has made significant strides toward understanding how the brain neurovascular unit accomplishes rapid and spatial increases in blood flow following neuronal activation. Among the experimental models used, the in vitro brain slice preparation provides unique information revealing the potential signals and cellular mechanisms involved in functional hyperemia. The most crucial limitation of this model, however, is the lack of intraluminal pressure and flow in the vessels being studied. Moreover, differences in basal vascular tone have led to varied interpretations regarding the polarity of vascular responses following neuron-to-glial stimulation. Given the complexity of astrocyte-induced neurovascular responses, we propose the use of a modified in vitro brain slice preparation, where intraluminal arteriolar pressure and flow are retained. Throughout this review, we discuss the advantages and disadvantages to be considered when using brain slices for neurovascular studies. Potential ways to overcome the current limitations are proposed.