How Rab proteins determine Golgi structure.
How Rab proteins determine Golgi structure.
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DOI：
10.1016/bs.ircmb.2014.12.002
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发表时间：
2015
影响因子：
--
通讯作者：
Storrie B
中科院分区：
文献类型：
--
作者：
Liu S;Storrie B
Rab蛋白（小GTPases）是哺乳动物高尔基体组织的关键调节因子。基于Rab激活状态的影响，Rab蛋白分为两种功能类别。在Class 1中，失活诱导高尔基带断裂和/或高尔基酶重新分布到ER，而野生型或激活的过表达对高尔基带组织几乎没有影响。在第二类中，情况正好相反。我们强调Rab 6，最丰富的高尔基体相关的Rab蛋白。Rab 6在HeLa细胞中的耗竭导致高尔基体池数量增加，更长，更连续的池，和囊泡的显著积累; Rab 6对高尔基体带组织的影响可能是通过调节囊泡运输。在效应器研究中，发现马达蛋白及其调节剂是Rab 6的关键效应器。一个相关的拉布，拉布41，影响高尔基带组织在一个对比的方式。负端和正端定向运动募集之间的平衡很可能是高尔基带组织中的主要Rab-dependent因素。
Rab proteins, small GTPases, are key regulators of mammalian Golgi apparatus organization. Based on the effect of Rab activation state, Rab proteins fall into two functional classes. In Class1, inactivation induces Golgi ribbon fragmentation and/or redistribution of Golgi enzymes to the ER, while overexpression of wild type or activation has little, if any, effect on Golgi ribbon organization. In Class 2, the reverse is true. We give emphasis to Rab6, the most abundant Golgi-associated Rab protein. Rab6 depletion in HeLa cells causes an increase in Golgi cisternal number, longer, more continuous cisternae, and a pronounced accumulation of vesicles; the effect of Rab6 on Golgi ribbon organization is probably through regulation of vesicle transport. In effector studies, motor proteins and their regulators are found to be key Rab6 effectors. A related Rab, Rab41, affects Golgi ribbon organization in a contrasting manner. The balance between minus- and plus-end directed motor recruitment may well be the major Rab-dependent factor in Golgi ribbon organization.