Mature Adult Dystrophic Mouse Muscle Environment Does Not Impede Efficient Engrafted Satellite Cell Regeneration and Self-Renewal
Mature Adult Dystrophic Mouse Muscle Environment Does Not Impede Efficient Engrafted Satellite Cell Regeneration and Self-Renewal
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DOI：
10.1002/stem.162
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发表时间：
2009-10-01
期刊：
影响因子：
5.2
通讯作者：
Morgan, Jennifer Elizabeth
中科院分区：
文献类型：
--
作者：
Boldrin, Luisa;Zammit, Peter Steven;Morgan, Jennifer Elizabeth
随着肌营养不良症的进展，骨骼肌环境发生的变化可能会影响干细胞功能，导致肌肉再生受损。以前有人认为，干细胞移植的成功可能因此取决于细胞本身的特性和宿主肌肉环境。在这里，我们移植年轻和成熟的成年mdx裸鼠，这是杜氏肌营养不良症的遗传同源物，与少量的卫星细胞新鲜分离的年轻，正常的供体小鼠。我们发现，供体卫星细胞有助于肌肉再生和自我更新，在成熟的成人，在年轻的，营养不良的宿主肌肉有效。供体来源的卫星细胞也有助于进一步损伤后的稳健再生，表明尽管存在更严重的营养不良肌肉环境，它们仍具有功能。这些发现提供了证据，表明干细胞可以有效地治疗营养不良后期的肌肉组织。干细胞2009; 27：2478 - 2487
Changes that occur in the skeletal muscle environment with the progress of muscular dystrophies may affect stem cell function and result in impaired muscle regeneration. It has previously been suggested that the success of stem cell transplantation could therefore be dependent both on the properties of the cell itself and on the host muscle environment. Here we engrafted young and mature adult mdx-nude mice, which are the genetic homolog of Duchenne muscular dystrophy, with a small number of satellite cells freshly isolated from young, normal donor mice. We found that the donor satellite cells contributed to muscle regeneration and self-renewal as efficiently within mature adult, as in young, dystrophic host muscle. Donor-derived satellite cells also contributed to robust regeneration after further injury, showing that they were functional despite the more advanced dystrophic muscle environment. These findings provide evidence that muscle tissue in a later stage of dystrophy may be effectively treated by stem cells. STEM CELLS 2009;27:2478-2487