Membrane receptors of mouse leukocytes. I. Two types of complement receptors for different regions of C3.
Membrane receptors of mouse leukocytes. I. Two types of complement receptors for different regions of C3.
复制标题
小鼠白细胞的膜受体。
作者：
E. Rabellino;G. D. Ross;M. Polley
用C包被红细胞（EAC）形成花环的方法研究了小鼠白细胞中第三种C组分的膜受体。设计了制备小鼠C3b或小鼠C3d EAC复合物的方法。EAC 1-3bmo用小鼠全血清处理，EAC 1-3bmo用含苏拉明钠的小鼠全血清处理。EAC复合物对小鼠白细胞的特异性通过液相人C3d抑制实验得到证实。低浓度的液相人C3d对EAC1-3dmo莲座有抑制作用，但对eac1 -3bmo莲座没有抑制作用。8倍浓度的流体相C3d可部分抑制EAC1-3bmo与淋巴细胞的花环形成，但对其他类型的小鼠白细胞无抑制作用。因此，小鼠的白细胞显然与人类和豚鼠的白细胞含有相同的两种C受体。小鼠CR1对C3b的非C3d区域具有特异性（可能类似于人类C3c），而小鼠CR2对C3b的C3d和C3d区域都具有特异性。
Mouse leukocytes were studied for membrane receptors for the third C component by rosette formation with C coated erythrocytes (EAC). Methods were devised for the preparation of EAC complexes containing either mouse C3b or mouse C3d. EAC 1-3dmo were prepared from EA treated with whole mouse serum while EAC 1-3bmo were produced from EAC 142hu treated with whole mouse serum containing sodium suramin. The specificity of the EAC complexes for mouse leukocytes was confirmed by inhibition experiments using fluid phase human C3d. Low concentrations of fluid phase human C3d inhibited EAC1-3dmo rosettes but failed to inhibit EAC 1-3bmo rosettes. Eight-fold higher concentrations of fluid phase C3d caused partial inhibition of EAC1-3bmo rosette formation with lymphocytes, but not with other types of murine leukocytes. Thus mouse leukocytes apparently contain the same two types of C receptors as do human and guinea pig leukocytes. Mouse CR1 is specific for a non-C3d region of C3b, (possibly analogous to human C3c) whereas mouse CR2 is specific for both C3d and the C3d region of C3b.