A solid state fungal fermentation-based strategy for the hydrolysis of wheat straw.
A solid state fungal fermentation-based strategy for the hydrolysis of wheat straw.
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DOI：
10.1016/j.biortech.2013.09.061
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发表时间：
2013-12
影响因子：
11.4
通讯作者：
Du C
中科院分区：
文献类型：
--
作者：
Pensupa N;Jin M;Kokolski M;Archer DB;Du C
将麦秆转化为水解产物的固态真菌发酵策略。通过在麦秆上培养黑曲霉来对麦秆进行生物预处理。使用麦秆作为主要底物生产 24.0 ± 1.76 U/g 纤维素酶。真菌提取物用于水解发酵的麦秆。使用新鲜制备的真菌提取物的水解效率比 Ctec2 高 19%。本文报道了一种基于固态真菌发酵的预处理策略，将小麦秸秆转化为可发酵的水解产物。首先在麦秆上培养黑曲霉以生产纤维素分解酶，然后麦秆被酶溶液水解成可发酵的水解产物。探索了提高纤维素酶产量的最佳含水量和三种麦秆改性方法。在水分含量为 89.5% 时，使用稀酸改性麦秆获得了 10.2 ± 0.13 U/g 纤维素酶活性。添加酵母提取物 (0.5% w/v) 和矿物质显着提高了纤维素酶的产量，达到 24.0 ± 1.76 U/g。使用真菌培养物滤液或商业纤维素酶 Ctec2 对发酵的麦秆进行水解，分别产生 4.34 和 3.13 g/L 的葡萄糖。这表明麦秆真菌发酵收获的真菌滤液含有比商业纤维素酶更合适的酶混合物。
A solid state fungal fermentation strategy converting wheat straw to hydrolysate. A biological pre-treatment of wheat straw by culturing A. niger on wheat straw. 24.0 ± 1.76 U/g cellulase was produced using wheat straw as the main substrate. The fungal extract was used to hydrolyze the fermented wheat straw. 19% higher hydrolysis efficiency using freshly-prepared fungal extract than Ctec2. This paper reports a solid-state fungal fermentation-based pre-treatment strategy to convert wheat straw into a fermentable hydrolysate. Aspergillus niger was firstly cultured on wheat straw for production of cellulolytic enzymes and then the wheat straw was hydrolyzed by the enzyme solution into a fermentable hydrolysate. The optimum moisture content and three wheat straw modification methods were explored to improve cellulase production. At a moisture content of 89.5%, 10.2 ± 0.13 U/g cellulase activity was obtained using dilute acid modified wheat straw. The addition of yeast extract (0.5% w/v) and minerals significantly improved the cellulase production, to 24.0 ± 1.76 U/g. The hydrolysis of the fermented wheat straw using the fungal culture filtrate or commercial cellulase Ctec2 was performed, resulting in 4.34 and 3.13 g/L glucose respectively. It indicated that the fungal filtrate harvested from the fungal fermentation of wheat straw contained a more suitable enzyme mixture than the commercial cellulase.