Cbf5p, the putative pseudouridine synthase of H/ACA-type snoRNPs, can form a complex with Gar1p and Nop10p in absence of Nhp2p and box H/ACA snoRNAs
Cbf5p, the putative pseudouridine synthase of H/ACA-type snoRNPs, can form a complex with Gar1p and Nop10p in absence of Nhp2p and box H/ACA snoRNAs
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DOI：
10.1261/rna.7770604
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发表时间：
2004-11-01
期刊：
影响因子：
4.5
通讯作者：
Caizergues-Ferrer, M
中科院分区：
文献类型：
--
作者：
Henras, AK;Capeyrou, R;Caizergues-Ferrer, M
盒C/D和盒H/ACA小核糖核蛋白颗粒（sRNP）被发现从古细菌到人类，其中一些在核糖体或剪接装置的组件的生物发生过程中发挥关键作用。这两种类型的颗粒的核心的蛋白质组成是很好地建立和盒C/D sRNP的组装途径已被广泛研究在古细菌和真核系统。相比之下，关于盒H/ACA sRNP的组装模式和最终结构的知识要有限得多。在本研究中，我们研究了蛋白质/蛋白质相互作用发生在四个蛋白质组分的酵母盒H/ACA小核仁RNP（snoRNP），Cbf 5 p，Gar 1 p，Nhp 2 p和Nop 10 p。我们提供的证据表明，Cbf 5 p，Gar 1 p和Nop 10 p可以形成一个没有Nhp 2 p和小核仁RNA（snoRNA）的颗粒成分的复合物，并且Cbf 5 p和Nop 10 p可以直接相互结合。我们还表明，盒H/ACA snoRNP组装所需的任何组件的情况下，抑制积累的Cbf 5 p，Car 1 p，或Nop 10 p，而Nhp 2 p水平几乎没有影响。
Box C/D and box H/ACA small ribonucleoprotein particles (sRNPs) are found from archaea to humans, and some of these play key roles during the biogenesis of ribosomes or components of the splicing apparatus. The protein composition of the core of both types of particles is well established and the assembly pathway of box C/D sRNPs has been extensively investigated both in archaeal and eukaryotic systems. In contrast, knowledge concerning the mode of assembly and final structure of box H/ACA sRNPs is much more limited. In the present study, we have investigated the protein/protein interactions taking place between the four protein components of yeast box H/ACA small nucleolar RNPs (snoRNPs), Cbf5p, Gar1p, Nhp2p, and Nop10p. We provide evidence that Cbf5p, Gar1p, and Nop10p can form a complex devoid of Nhp2p and small nucleolar RNA (snoRNA) components of the particles and that Cbf5p and Nop10p can directly bind to each other. We also show that the absence of any component necessary for assembly of box H/ACA snoRNPs inhibits accumulation of Cbf5p, Car1p, or Nop10p, whereas Nhp2p levels are little affected.