Analyses of long non-coding RNAs and mRNA profiling through RNA sequencing of MDBK cells at different stages of bovine viral diarrhea virus infection
Analyses of long non-coding RNAs and mRNA profiling through RNA sequencing of MDBK cells at different stages of bovine viral diarrhea virus infection
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通过牛病毒性腹泻病毒感染不同阶段MDBK细胞的RNA测序分析长非编码RNA和mRNA谱
DOI：
10.1016/j.rvsc.2017.09.020
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发表时间：
2017-12-01
影响因子：
2.4
通讯作者：
Ni, Wei
中科院分区：
文献类型：
--
作者：
Ma, Qiman;Li, Liangyuan;Ni, Wei
背景资料：牛病毒性腹泻病毒（BVDV）感染是一个动态和复杂的过程，导致奶牛业和养牛业的重大经济损失。方法：通过对BVDV NADL感染过程中长链非编码RNA（IncRNA）表达的深度测序，研究BVDV NADL感染过程中长链非编码RNA（IncRNA）的表达，以确定BVDV是否调控宿主细胞的特异性活性。在感染后2 h、6 h和18 h，共有1236个lncRNA转录本和3261个mRNA转录本受到差异调节。lncRNA在外显子长度、数量、表达水平和保守性方面与其他哺乳动物具有相同的特征。GO富集和KEGG途径分析表明，lncRNA在BVDV感染过程中调节免疫反应。13个差异表达的基因在18 hpi的选择和独立验证的逆转录qPCR.Conclusions：本研究是第一个提供的见解lncRNA和BVDV的生物学连接，可以进一步探讨抗病毒预防策略的发展，并在理解病毒和宿主成分之间的持续感染。
Background: Bovine viral diarrhea virus (BVDV) infection is a dynamic and complex process that leads to significant economic losses in the dairy and cattle industries. However, our understanding of the protective and pathological mechanism underlying host infection is limited.Methods: To determine whether BVDV regulates specific activities of the host cell, the expression of long non coding RNA (IncRNA) during BVDV NADL infection was studied by deep sequencing.Results: A total of 1236 lncRNA transcripts and 3261 mRNA transcripts were differentially regulated at 2 h, 6 h, and 18 h post-infection. The lncRNAs shared same characteristics with other mammals in terms of exon length, number, expression level, and conservation. The Gene Ontology (GO) enrichment and KEGG pathway analyses showed that lncRNAs regulate immune reaction during BVDV infection. Thirteen differentially expressed genes in 18 hpi were selected and independently validated by reverse-transcription qPCR.Conclusions: The present study is the first to provide insights into the biological connection of lncRNAs and BVDV, which can be further explored for the development of antiviral prevention strategies and in understanding persistent infection between viral and host components.