An enzymatically active a domain is required for cholera-like enterotoxins to induce a long-lived blockade on the induction of oral tolerance: new method for screening mucosal adjuvants.
An enzymatically active a domain is required for cholera-like enterotoxins to induce a long-lived blockade on the induction of oral tolerance: new method for screening mucosal adjuvants.
复制标题
霍乱样肠毒素需要具有酶活性的 a 结构域来诱导口服耐受的长期阻断：筛选粘膜佐剂的新方法。
DOI：
10.1128/iai.71.12.6850-6856.2003
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发表时间：
2003
影响因子：
3.1
通讯作者：
Lewis,GeorgeK
中科院分区：
文献类型：
--
作者：
Bagley,KennethC;Abdelwahab,SayedF;Tuskan,RobertG;Lewis,GeorgeK
The cholera-like enterotoxins (CLETS), cholera toxin (CT) andEscherichia coliheat-labile toxin (LT), are powerful mucosal adjuvants. Here we show that these toxins also induce a long-lived blockade (of at least 6 months) on the induction of oral tolerance when they are coadministered with the antigen ovalbumin. Strikingly, only enzymatically active CLETS induced this blockade on the induction of oral tolerance. In this regard, the enzymatically inactive mutants of CT and LT, CTK63 and LTK63, and their recombinant B pentamers, rCTB and rLTB, failed to block the induction of oral tolerance, demonstrating a stringent requirement for an enzymatically active A domain in this phenomenon. Together with the results of other recent studies, these results indicate that the enzymatic activity of CLETS, most likely cyclic AMP elevation, is responsible for their adjuvant effects. The results of this study also indicate that measuring the ability of putative mucosal adjuvants to block the induction of oral tolerance may be a superior method for measuring mucosal adjuvanticity.
