Cellular architecture of the nucleus reuniens thalami and its putative aspartatergic/glutamatergic projection to the hippocampus and medial septum in the rat
Cellular architecture of the nucleus reuniens thalami and its putative aspartatergic/glutamatergic projection to the hippocampus and medial septum in the rat
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DOI：
10.1046/j.1460-9568.2002.02189.x
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发表时间：
2002-10-01
影响因子：
3.4
通讯作者：
Kiss, J
中科院分区：
文献类型：
--
作者：
Bokor, H;Csáki, A;Kiss, J
很少有人知道的神经化学特征的丘脑连合核（RE）。在本研究中，进行免疫细胞化学实验，以表征某些神经化学标志物的表达模式，例如钙结合蛋白钙结合蛋白和钙视网膜蛋白和几种神经肽。共定位的研究表明，一半的钙结合蛋白阳性细胞表达calretinin，许多calretinin免疫反应神经元含有calbindin。与此相反，免疫标记的神经肽并没有揭示RE中的细胞体。RE与几个边缘结构建立了广泛的联系。将这些投射模式与RE神经元的神经化学特征相关联，将逆行示踪剂[H-3] D-天冬氨酸（其选择性地由使用谷氨酸作为神经递质的高亲和力摄取位点摄取）和非选择性逆行示踪剂麦胚凝集素结合的胶体金注射到海马CA 1子域的腔隙分子层和内侧隔中。结果提供了从RE到海马和到内侧隔的交感神经/交感神经投射的直接解剖学证明。几乎所有的投射神经元被证明是钙结合蛋白免疫阳性，其中许多表达calretinin。两种逆行标记技术显示，投射到海马的神经元位于RE的背外侧部分的集群，而投射到内侧隔的神经元主要分布在核的腹内侧部分，表明不同的细胞群投射到这些边缘区。这些结果表明，RE中的神经元是异质性的，并有助于隔-海马系统的兴奋性神经支配。
Little is known about the neurochemical features of the nucleus reuniens thalami (RE). In the present study, immunocytochemical experiments were performed to characterize the expression pattern of certain neurochemical markers, e.g. the calcium-binding proteins calbindin and calretinin and several neuropeptides. Colocalization studies revealed that half of the calbindin-positive cells express calretinin, and numerous calretinin-immunoreactive neurons contain calbindin. In contrast, immunolabelling for neuropeptides did not reveal cell bodies in the RE. The RE establishes widespread connections with several limbic structures. To correlate these projection patterns with the neurochemical characteristics of RE neurons, the retrograde tracer [H-3]D-aspartate, which is selectively taken up by high affinity uptake sites that use glutamate as neurotransmitter, and the nonselective retrograde tracer wheatgerm agglutinin-conjugated colloidal gold was injected into the stratum lacunosum moleculare of the hippocampal CA1 subfield and into the medial septum. The results provide direct anatomical demonstration of aspartatergic/glutamatergic projection from the RE to the hippocampus and to the medial septum. Nearly all of the projecting neurons proved to be calbindin-immunopositive and many of them expressed calretinin. Both retrograde labelling techniques revealed that neurons projecting to the hippocampus were located in clusters in the dorsolateral part of the RE, whereas neurons projecting to the medial septum were mainly distributed in the ventromedial portion of the nucleus, indicating that different cell populations project to these limbic areas. These results suggest that neurons in the RE are heterogeneous and contribute to the excitatory innervation of the septo-hippocampal system.