High-fidelity mass analysis unveils heterogeneity in intact ribosomal particles
High-fidelity mass analysis unveils heterogeneity in intact ribosomal particles
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DOI：
10.1038/nmeth.4147
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发表时间：
2017-03-01
期刊：
影响因子：
48
通讯作者：
Heck, Albert J. R.
中科院分区：
文献类型：
--
作者：
van de Waterbeemd, Michiel;Fort, Kyle L.;Heck, Albert J. R.
研究蛋白质-核酸大分子机器的结构、组装和功能需要多维分子和结构生物学方法。我们描述了对Orbitrap质谱仪的改进，使0.8-到2.3-丙二醛原核生物30S、50S和70S核糖体颗粒和9-丙二醛鸡舍病毒的高分辨率自然MS分析成为可能。该仪器改进的质量范围和灵敏度很容易暴露核糖体相关蛋白SRA的意外结合。
Investigation of the structure, assembly and function of protein-nucleic acid macromolecular machines requires multidimensional molecular and structural biology approaches. We describe modifications to an Orbitrap mass spectrometer, enabling high-resolution native MS analysis of 0.8- to 2.3-MDa prokaryotic 30S, 50S and 70S ribosome particles and the 9-MDa Flock House virus. The instrument's improved mass range and sensitivity readily exposes unexpected binding of the ribosome-associated protein SRA.