The use of radionuclide angiography to study blood flow through endothelial cell seeded extrathoracic bypass grafts in the dog.
The use of radionuclide angiography to study blood flow through endothelial cell seeded extrathoracic bypass grafts in the dog.
复制标题
使用放射性核素血管造影来研究狗体内接种内皮细胞的胸腔外搭桥移植物的血流。
DOI：
10.1111/j.1740-8261.1997.tb00830.x
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
LothropJr,CD
中科院分区：
文献类型：
--
作者：
Sackman,JE;Daniel,GB;Freeman,MB;LothropJr,CD
血管移植物的内皮种植已被证明可以降低移植物的血栓形成性，并延长植入体内时的寿命。以前的研究利用解剖移植研究内皮化和愈合。解剖型胸腹部移植物不允许对单个移植物进行连续活检评估，也不接近用于肢体挽救的长旁路移植物的环境。本研究评价了使用解剖外主动脉旁路移植物评估内皮细胞接种的膨体聚四氟乙烯（ePTFE）的愈合情况。放射性核素血管造影用于评估移植物通畅性和量化通过移植物的血流量。对犬放置了一个长60 cm、内径8 mm的解剖外移植物，植入自体内皮。移植物从2周至1年进行活检。在植入后和每次移植物活检后进行放射性核素研究。所有犬的移植物放置和活检耐受性良好。移植物的活检片段允许通过扫描电子显微镜和光学显微镜对植入移植物的愈合进行连续研究。通过植入移植物的流量接近尾侧腹主动脉总流量的50%。无论是动物之间还是随时间推移，移植物流量均无显著差异。
Endothelial seeding of vascular grafts has been shown to decrease graft thrombogenicity and prolong longevity when implanted in vivo. Previous studies have utilized anatomic grafts to study endothelialization and healing. Anatomic thoracoabdominal grafts do not allow for sequential biopsy for evaluation of individual grafts nor do they approximate the environment for long bypass grafts used in limb salvage. This study evaluated the use of an extra‐anatomic aortic bypass graft to assess the healing of endothelial cell seeded expanded polytetrafluoroethylene (ePTFE). Radionuclide angiography was used to evaluate graft patency and quantify blood flow through the graft. Dogs underwent placement of an extra‐anatomic 60 cm long, 8 mm internal diameter, graft seeded with autologous endothelium. Grafts were biopsied from 2 weeks up to 1 year. Radionuclide studies were performed postimplantation and following each graft biopsy. Graft placement and biopsies were well tolerated in all dogs. Biopsied segments of graft allowed for sequential studies of the healing of implanted grafts by scanning electron and light microscopy. Flow through the implanted graft was close to 50% of the total caudal abdominal aortic flow. No significant difference in graft flow was noted either between animals or overtime.