Morphological and molecular characterization of Parafurgasonia zhangi spec. nov. and Chilodonella acuta Kahl, 1931 (Protozoa, Ciliophora), from a soil habitat of Saudi Arabia.
Morphological and molecular characterization of Parafurgasonia zhangi spec. nov. and Chilodonella acuta Kahl, 1931 (Protozoa, Ciliophora), from a soil habitat of Saudi Arabia.
复制标题
DOI：
10.1099/ijs.0.062026-0
复制
发表时间：
2014-07
影响因子：
2.8
通讯作者：
Xinpeng Fan;Rui Ma;S. Al‐Farraj;F. Gu
中科院分区：
文献类型：
--
作者：
Xinpeng Fan;Rui Ma;S. Al‐Farraj;F. Gu
本文报道了采自沙特阿拉伯的两种土壤纤毛虫Parafurgasonia zhangi spec. nov.和Chilodonella acuta Kahl，1931的形态和纤毛下结构。张氏拟步行虫与其同类的区别在于：排泄孔位于口旁膜的后端，背侧有16或17个体动子，每一个体动子约有11个动子，口旁膜微弯曲，由约15个双动子组成，口下器由4或5列动子组成，每列动子有4个单动子。对尖突奇齿线虫的诊断进行了更新，包括银浸渍法显示的特征：体内细胞大小为33-45 × 18-26 µm，存在背驼峰和尾状足，两个收缩泡，7个左侧和5个右侧动力，9-11根线虫连接杆，背刷含有约11个基体。基于小亚基rRNA基因序列的系统发育分析表明，张氏棘齿象与以布氏棘齿象为代表的棘齿象科的种比较接近，而尖齿象与钩齿象聚在一起，但属于轮廓清晰的种。
The morphology and infraciliature of two soil ciliates, Parafurgasonia zhangi spec. nov. and Chilodonella acuta Kahl, 1931, collected from Saudi Arabia, were investigated by observations of both living cells and specimens after standard staining methods. P. zhangi differs from its congeners by the combination of the following features: excretory pore quite near posterior end of paroral membrane, 16 or 17 somatic kineties with about 11 kinetids in each one on dorsal side, paroral membrane gently curved and composed of about 15 dikinetids, and hypostomial organelle composed of four or five files of kinetids with four monokinetids each. The diagnosis of Chilodonella acuta was renewed to include characteristics revealed by the silver impregnation method: cells in vivo measuring 33-45) × 18-26) µm, dorsal hump and tail-like podite present, two contractile vacuoles, seven left and five right kineties, 9-11 nematodesmal rods, and dorsal brush containing about 11 basal bodies. Phylogenetic analyses based on small-subunit rRNA gene sequences showed that P. zhangi was closer to species of the Colpodidiidae rather than the Furgasoniidae represented by Furgasonia blochmanni, and Chilodonella acuta clustered with its congener Chilodonella uncinata but was a well-outlined species of the genus.