Exploring the Microbial Ecological Functions in Response to Vertical Gradients in a Polluted Urban River
Exploring the Microbial Ecological Functions in Response to Vertical Gradients in a Polluted Urban River
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DOI：
10.1002/clen.202100004
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发表时间：
2021-08
期刊：
影响因子：
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通讯作者：
Wei Cai;Yi Li;Jian Hu;Haomiao Cheng
中科院分区：
文献类型：
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作者：
Wei Cai;Yi Li;Jian Hu;Haomiao Cheng
陡峭的垂直环境梯度可能会不同地影响微生物的结构和功能。在这里，作者提供了一些利用GeoChip 5.0微阵列对污染的城市河流中微生物功能的垂直分布的首次见解。在中国境内的金川河中，共检测到1213类89615个功能基因。涉及金属动态平衡、胁迫反应、碳循环、抗生素抗性和有机污染物降解的基因是主要的基因类型，分别占基因总数的24.63%、15.20%、14.17%、10.59%和8.03%。不同深度的功能基因组丰度没有显著差异。不同的基因类别在不同的深度显示其代谢潜力的微小波动。底层和沉积层的功能基因多样性高于表层。此外，水中的抗生素抗性、有机污染物降解和色素基因的功能潜力比沉积物中的更强，而氮循环和微生物防御的功能潜力随着深度的增加而增加。表层沉积物和表层水层分别具有最大的碳循环和有机污染物降解过程的代谢潜力。中层沉积物具有最大的氮磷循环潜力。
Sharp vertical environmental gradients could differentially affect the structure and function of microbes. Here, the authors provide some of the first insights into the vertical distribution of microbial functions in a polluted urban river using Geochip 5.0 microarray. A total of 89 615 functional genes belonging to 1213 categories are detected in Jinchuan River in China. Genes involved in metal homeostasis, stress response, carbon cycling, antibiotic resistance, and organic contaminant degradation are the primary gene categories detected and comprise 24.63%, 15.20%, 14.17%, 10.59%, and 8.03% of total genes. There are no significant differences in the abundance of functional gene groups at varying depths. Different gene categories showed small fluctuations in their metabolic potentials at varying depths. Functional gene diversity in bottom water and sediment layers is higher than in surface layers. Additionally, functional potentials of antibiotic resistance, organic contaminant degradation, and pigment genes are stronger in water than in sediment, while those of nitrogen cycling and microbial defense increased with depth. The surface sediment layer and surface water layer have the greatest metabolic potential for the carbon cycle and organic pollutant degradation processes, respectively. The middle sediment layer has the highest potential for the nitrogen and phosphorus cycles.