Comprehensive characterization of protein 4.1 expression in epithelium of large intestine
Comprehensive characterization of protein 4.1 expression in epithelium of large intestine
复制标题
大肠上皮蛋白4.1表达的综合表征
DOI：
10.1007/s00418-014-1224-z
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发表时间：
2014
影响因子：
2.3
通讯作者：
X. An
中科院分区：
文献类型：
--
作者：
Jingxin Zhang;Shaomin Yang;Chao An;Jie Wang;Hongxia Yan;Yumin Huang;Jinlei Song;Chang;A. Baines;N. Mohandas;X. An
蛋白质 4.1 家族由四个成员组成：4.1R、4.1N、4.1B 和 4.1G，每个成员由不同的基因编码。所有 4.1 mRNA 均经历广泛的选择性剪接。从功能上讲，它们通常充当将基于肌动蛋白的细胞骨架连接到质膜蛋白的接头。据报道，4.1蛋白在大多数动物细胞类型和组织中表达，包括上皮细胞和上皮组织。然而，4.1蛋白在大肠中的表达尚未得到很好的表征。在本研究中，我们进行了RT-PCR、蛋白质印迹和免疫组织化学分析来表征小鼠大肠上皮细胞中4.1蛋白的转录本、蛋白表达和细胞定位。我们发现每个基因衍生出多个转录本，包括八个 4.1R 亚型、四个 4.1N 亚型、四个 4.1B 亚型和六个 4.1G 亚型。然而，在蛋白质水平上，仅检测到一两个主要条带，这意味着并非所有转录本都被翻译和/或蛋白质没有以可检测的水平积累。免疫组织化学显示，4.1R、4.1N 和 4.1B 均在上皮细胞的侧膜和细胞质中表达，表明这些蛋白的作用可能存在冗余。我们的研究结果不仅为蛋白质4.1基因的结构提供了新的见解，而且为未来的功能研究奠定了基础。
The protein 4.1 family consists of four members, 4.1R, 4.1N, 4.1B and 4.1G, each encoded by a distinct gene. All 4.1 mRNAs undergo extensive alternative splicing. Functionally, they usually serve as adapters that link actin-based cytoskeleton to plasma membrane proteins. It has been reported that 4.1 proteins are expressed in most animal cell types and tissues including epithelial cells and epithelial tissues. However, the expression of 4.1 proteins in large intestine has not been well characterized. In the present study, we performed RT-PCR, western blot and immunohistochemistry analysis to characterize the transcripts, the protein expression and cellular localization of 4.1 proteins in the epithelia of mouse large intestine. We show that multiple transcripts derive from each gene, including eight 4.1R isoforms, four 4.1N isoforms, four 4.1B isoforms and six 4.1G isoforms. However, at the protein level, only one or two major bands were detected, implying that not all transcripts are translated and/or the proteins do not accumulate at detectable levels. Immunohistochemistry revealed that 4.1R, 4.1N and 4.1B are all expressed at the lateral membrane as well as cytoplasm of epithelial cells, suggesting a potentially redundant role of these proteins. Our findings not only provide new insights into the structure of protein 4.1 genes but also lay the foundation for future functional studies.
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DOI：
10.1016/s0021-9258(18)92973-x
发表时间：
1991-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Conboy;J. Chan;J. Chasis;Y. Kan;N. Mohandas
通讯作者：
J. Conboy;J. Chan;J. Chasis;Y. Kan;N. Mohandas
膜骨架蛋白 4.1 中选择性剪接的血影蛋白-肌动蛋白结合域的机械化学。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Discher,D;Parra,M;Conboy,JG;Mohandas,N
通讯作者：
Mohandas,N
影响因子：
19.6
作者：
Ramez, M;Blot-Chabaud, M;Gascard, P
通讯作者：
Gascard, P
影响因子：
2.9
作者：
Gimm, JA;An, XL;Mohandas, N
通讯作者：
Mohandas, N
影响因子：
20.3
作者：
Kang, Qiaozhen;Yu, Yu;An, Xiuli
通讯作者：
An, Xiuli