Reversible and selective luminescent determination of ClO-/H2S redox cycle in vitro and in vivo based on a ruthenium trisbipyridyl probe
Reversible and selective luminescent determination of ClO-/H2S redox cycle in vitro and in vivo based on a ruthenium trisbipyridyl probe
复制标题
基于钌三联吡啶探针可逆选择性发光测定体外和体内 ClO-/H2S 氧化还原循环
DOI：
10.1039/c4an00331d
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发表时间：
2014-01-01
期刊：
影响因子：
4.2
通讯作者：
Sun, Shiguo
中科院分区：
文献类型：
--
作者：
Liu, Fengyu;Gao, Yulong;Sun, Shiguo
一种快速、高选择性的荧光探针已被开发出来，用于测定体内和体外的ClO-/H_2S氧化还原循环，它使用与吩噻嗪共价连接的Ru-三联吡啶配合物。加入ClO-后，荧光强度大大增强，这是因为探针被氧化成其亚砜的衍生物，由于探针的重组，通过与硫化氢的反应，该衍生物很快恢复到原来的水平。氧化还原循环至少可以重复12次。在最佳条件下，ClO-在1×10~(-9)~1×10~(-4)mol·L~(-1)和H_2S在1×10~(-9)~1×10~(-4)mol·L~(-1)浓度范围内呈良好的线性关系，检出限分别为1.8×10~(-11)mol·L~(-1)和1.2×10~(-11)mol·L~(-1)，远低于其他检测方法。该方法设计简单，操作快速，适用于体内和体外ClO-和H_2S的可逆测定，具有较高的选择性。
A rapid and highly selective luminescent probe has been developed to determine the in vitro and in vivo ClO-/H2S redox cycle using a ruthenium tris-bipyridyl complex covalently linked with phenothiazine. The luminescence intensity was considerably enhanced upon the addition of ClO- due to the oxidation of the probe to its sulfoxide derivative, which quickly returned to the original level by the reaction with H2S due to the reconstitution of the probe. The redox cycle can be repeated at least 12 times. Under optimal conditions, the luminescence intensities are linear over the concentration range of 1 x 10(-9) to 1 x 10(-4) mol L-1 for ClO- and 1 x 10(-9) to 1 x 10(-4) mol L-1 for H2S, and the detection limits are 1.8 x 10(-11) mol L-1 for ClO- and 1.2 x 10(-11) mol L-1 for H2S, which are much lower than those obtained with other detection methods. The proposed method is simple in design and fast in operation, and is suitable for the reversible determination of ClO- and H2S in vitro and in vivo with high selectivity.