Baicalein inhibits lipid accumulation by regulating early adipogenesis and m-TOR signaling
Baicalein inhibits lipid accumulation by regulating early adipogenesis and m-TOR signaling
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DOI：
10.1016/j.fct.2014.02.009
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发表时间：
2014-05-01
影响因子：
4.3
通讯作者：
Lee, Boo-Yong
中科院分区：
文献类型：
--
作者：
Seo, Min-Jung;Choi, Hyeon-Son;Lee, Boo-Yong
黄芩素是一种来源于黄芩的黄酮类化合物。在这项研究中，我们研究了黄芩素如何抑制3 T3-L1细胞脂肪形成过程中的脂质积累。我们的数据表明，黄芩素以剂量依赖性方式抑制脂肪形成过程中的脂质积累。黄芩素的抑制作用仅限于早期成脂阶段。细胞周期分析显示黄芩素通过下调cyclin的表达，使细胞周期阻滞于GO/G1期。此外，黄芩素抑制早期脂肪形成因子的mRNA表达，导致晚期脂肪形成因子在mRNA和蛋白水平上下调。黄芩素对成脂因子的抑制作用与脂质合成酶的下调有关。此外，黄芩素负性调节参与脂肪形成过程中脂质积累的m-TOR信号通路，从而抑制m-TOR和p70 S6 K的磷酸化。在一项斑马鱼研究中，黄芩素显著降低了尼罗红染色中的脂质积累。与使用细胞系的报告一致，黄芩素以剂量依赖性方式降低成脂因子的mRNA表达。该结果反映了基于甘油三酯测定的总甘油三酯水平降低。我们的数据表明，黄芩素通过控制3 T3-L1细胞的细胞周期和m-TOR信号来抑制脂质积聚，并且在斑马鱼模型中发现了其抗脂肪形成作用。(C)2014爱思唯尔有限公司版权所有。
Baicalein is a type of flavonoid that originates from Scutellaria baicalensis. In this study, we examined how baicalein inhibits lipid accumulation during adipogenesis in 3T3-L1 cells. Our data show that baicalein inhibited lipid accumulation during adipogenesis in a dose-dependent manner. Baicalein inhibition was limited to the early adipogenic stage. Cell cycle analysis showed that baicalein induced cell cycle arrest in the GO/G1 phase through cyclin downregulation. In addition, baicalein suppressed the mRNA expression of early adipogenic factors leading to downregulation of late adipogenic factors at mRNA and protein levels. Inhibition of adipogenic factors by baicalein was correlated with downregulation of lipid synthetic enzymes. Additionally, baicalein negatively regulated the m-TOR signaling pathway involved in lipid accumulation during adipogenesis, thus inhibiting phosphorylation of m-TOR and p70S6 K. In a zebrafish study, baicalein significantly reduced lipid accumulation in Nile Red staining. Consistent with a report using cell lines, mRNA expression of adipogenic factors was decreased in a dose-dependent manner by baicalein. This result reflects a reduction in total triglyceride levels based on a triglyceride assay. Our data suggest that baicalein inhibits lipid accumulation by controlling the cell cycle and m-TOR signaling in 3T3-L1 cells, and its anti-adipogenic effect was found in a zebrafish model. (C) 2014 Elsevier Ltd. All rights reserved.