A Combined golgi and postembedding GABA and glutamate electron microscopic study of the nucleus dorsomedialis thalami of the rat.
A Combined golgi and postembedding GABA and glutamate electron microscopic study of the nucleus dorsomedialis thalami of the rat.
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大鼠丘脑背内侧核的高尔基体和嵌入后 GABA 和谷氨酸盐的联合电子显微镜研究。
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
J. Hámori
中科院分区：
文献类型：
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作者：
László Négyessy;J. Takács;I. Divac;J. Hámori
采用高尔基法和包埋后γ -氨基丁酸（GABA）和/或谷氨酸（GLU）免疫金联合方法研究背内侧核（MD）神经元的突触组织。MD中浸渍神经元的形态特征（Kuroda et al. 1992a详细描述）与其他丘脑核中存在的丘脑皮质中继细胞相似。找不到gaba能细胞体。金色调神经元的远端树突状分支与中小尺寸谷氨酸以及gaba免疫反应性轴突末梢建立了突触接触。大的谷氨酸和gaba阳性轴突末端与近端树突形成突触接触。gaba能轴突末端具有对称的突触接触和多形性囊泡。谷氨酸免疫反应末端形成不对称突触并含有球形囊泡，尽管一些大的谷氨酸免疫反应末端含有多形性囊泡。突触肾小球样复合物也可以观察到谷氨酸和gaba阳性的大轴突末端与高尔基体浸染的近端树突突触的参与。
The synaptic organisation of neurons in the nucleus medialis dorsalis (MD) was investigate by combined Golgi, and post-embedding gamma-aminobutyric acid (GABA) and/or glutamate (GLU) immunogold methods. The morphological features of the impregnated neurons in the MD (detailed by Kuroda et al. 1992a) were similar to thalamocortical relay cells present in other thalamic nuclei. GABAergic cell bodies could not be found. Distal dendritic branches of gold toned neurons established synaptic contacts with small and medium size glutamate as well as GABA-immunoreactive axonal endings. Large glutamate and GABA-positive axon terminals formed synaptic contacts with proximal dendrites. GABAergic axon terminals exhibited symmetric synaptic contacts and contained pleomorphic vesicles. Glutamate immunoreactive terminals formed asymmetric synapses and contained spheroid vesicles, although, some large glutamate immunoreactive endings contained pleomorphic vesicles. Synaptic glomerulus-like complexes could also be observed with the participation of glutamate-, and GABA-positive large axon terminals synapsing with Golgi impregnated proximal dendrites.