High throughput 16SrRNA gene sequencing reveals the correlation between Propionibacterium acnes and sarcoidosis.
High throughput 16SrRNA gene sequencing reveals the correlation between Propionibacterium acnes and sarcoidosis.
复制标题
高通量16SrRNA基因测序揭示痤疮丙酸杆菌与结节病之间的相关性
DOI：
10.1186/s12931-017-0515-z
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发表时间：
2017-02-01
影响因子：
5.8
通讯作者：
Li HP
中科院分区：
文献类型：
--
作者：
Zhao MM;Du SS;Li QH;Chen T;Qiu H;Wu Q;Chen SS;Zhou Y;Zhang Y;Hu Y;Su YL;Shen L;Zhang F;Weng D;Li HP
本研究旨在利用高通量16 SrRNA基因测序技术检测结节病患者淋巴结活检标本的细菌谱，进一步验证痤疮丙酸杆菌与结节病的相关性。方法收集纵隔淋巴结活检标本36例，其中结节病17例，结核8例，淋巴结转移8例非感染性肺部疾病11例（对照组）。采用高通量测序平台MiSeq对标本中细菌16 SrRNA基因V4区进行扩增和测序，建立细菌谱。结果共鉴定出545个菌属，结节病组中38个菌属的相对丰度显著低于结核组和对照组，29个菌属的相对丰度显著高于结核组和对照组（P< 0.01）。痤疮丙酸杆菌16 SrRNA仅在结节病组的17例标本中检出，而在结核病组和对照组中均未检出。结节病组痤疮丙酸杆菌相对丰度（0. 16% ± 0. 15%）高于正常对照组（0. 16% ± 0. 15%）。11%）显著高于结核组（P= 0.0010，q = 0.0044）和对照组（P= 0.0010，q = 0.0038）。颗粒青霉相对丰度仅为0.0022% ± 0.结节病组为00 44%。结论高通量16 SrRNA基因测序技术可用于结节病标本的细菌学研究。结果表明，痤疮丙酸杆菌可能参与结节病的发展。
ObjectiveThis study aims to use high throughput 16SrRNA gene sequencing to examine the bacterial profile of lymph node biopsy samples of patients with sarcoidosis and to further verify the association between Propionibacterium acnes (P. acnes) and sarcoidosis.MethodsA total of 36 mediastinal lymph node biopsy specimens were collected from 17 cases of sarcoidosis, 8 tuberculosis (TB group), and 11 non-infectious lung diseases (control group). The V4 region of the bacterial 16SrRNA gene in the specimens was amplified and sequenced using the high throughput sequencing platform MiSeq, and bacterial profile was established. The data analysis software QIIME and Metastats were used to compare bacterial relative abundance in the three patient groups.ResultsOverall, 545 genera were identified; 38 showed significantly lower and 29 had significantly higher relative abundance in the sarcoidosis group than in the TB and control groups (P< 0.01). P. acnes 16SrRNA was exclusively found in all the 17 samples of the sarcoidosis group, whereas was not detected in the TB and control groups. The relative abundance of P. acnes in the sarcoidosis group (0.16% ± 0. 11%) was significantly higher than that in the TB (Metastats analysis:P= 0.0010, q = 0.0044) and control groups (Metastats analysis:P= 0.0010, q = 0.0038). The relative abundance of P. granulosum was only 0.0022% ± 0. 0044% in the sarcoidosis group. P. granulosum 16SrRNA was not detected in the other two groups.ConclusionHigh throughput 16SrRNA gene sequencing appears to be a useful tool to investigate the bacterial profile of sarcoidosis specimens. The results suggest that P. acnes may be involved in sarcoidosis development.