Schistosoma mansoni venom allergen-like proteins: phylogenetic relationships, stage-specific transcription and tissue localization as predictors of immunological cross-reactivity
Schistosoma mansoni venom allergen-like proteins: phylogenetic relationships, stage-specific transcription and tissue localization as predictors of immunological cross-reactivity
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DOI：
10.1016/j.ijpara.2019.03.003
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发表时间：
2019-07-01
影响因子：
4
通讯作者：
Hoffmann, Karl F.
中科院分区：
文献类型：
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作者：
Farias, Leonardo P.;Chalmers, Lain W.;Hoffmann, Karl F.
曼氏血吸虫毒液类变应原蛋白(SmVALs)是一个不同的蛋白质超家族的一部分，分为两组(组1和组2)。对第1组SmVALs的系统发育分析表明，SmVALs的成员可分为亚支(A-D)；这些亚支在寄生虫的整个生命周期和免疫交叉反应中拥有相似的基因表达模式。此外，整装原位杂交表明，与系统发育、转录和免疫相关的SmVAL4、10、18和19(C亚类)均定位于未成熟尾蚴的髋臼前腺体。我们的结果表明，SmVAL组1的系统发育关系、阶段特异性转录图谱和组织定位可以预测免疫交叉反应。(C)2019年提交人。由爱思唯尔有限公司代表澳大利亚寄生虫学学会出版。
Schistosoma mansoni venom allergen-like proteins (SmVALs) are part of a diverse protein superfamily partitioned into two groups (group 1 and group 2). Phylogenetic analyses of group 1 SmVALs revealed that members could be segregated into subclades (A-D); these subclades share similar gene expression patterns across the parasite lifecycle and immunological cross-reactivity. Furthermore, whole-mount in situ hybridization demonstrated that the phylogenetically, transcriptionally and immunologically-related SmVAL4, 10, 18 and 19 (subclade C) were all localized to the pre-acetabular glands of immature cercariae. Our results suggest that SmVAL group 1 phylogenetic relationships, stage-specific transcriptional profiles and tissue localization are predictive of immunological cross-reactivity. (C) 2019 The Authors. Published by Elsevier Ltd on behalf of Australian Society for parasitology.