Genetic evidence for functional interactions between actin noncomplementing (Anc) gene products and actin cytoskeletal proteins in Saccharomyces cerevisiae.
Genetic evidence for functional interactions between actin noncomplementing (Anc) gene products and actin cytoskeletal proteins in Saccharomyces cerevisiae.
复制标题
酿酒酵母中肌动蛋白非互补 (Anc) 基因产物和肌动蛋白细胞骨架蛋白之间功能相互作用的遗传证据。
DOI：
10.1093/genetics/135.2.275
复制
发表时间：
1993
期刊：
影响因子：
3.3
通讯作者：
Drubin,DG
中科院分区：
文献类型：
--
作者：
Vinh,DB;Welch,MD;Corsi,AK;Wertman,KF;Drubin,DG
我们在这里描述的肌动蛋白非互补（ANC）基因和肌动蛋白（ACT 1）或肌动蛋白结合蛋白（SAC6，ABP1，TPM1）基因的突变等位基因之间的遗传相互作用。anc突变被发现与不同的act1突变表现出等位基因特异性的非互补相互作用。此外，还检测了4个ANC基因（ANC 1、ANC 2、ANC 3和ANC 4）的突变等位基因与肌动蛋白结合蛋白基因的无效等位基因的相互作用。一个anc1突变等位基因未能补充无效等位基因的SAC6和TPM1基因编码的酵母fimplant和原肌球蛋白，分别。此外，还观察到anc3和sac6突变之间以及anc4和tpm1突变之间的合成致死性。综上所述，上述结果强烈表明ANC基因产物有助于肌动蛋白功能的各个方面。最后，我们报告的两个模型先前提出的解释extragenic noncomplementation的测试结果。
We describe here genetic interactions between mutant alleles of Actin-NonComplementing (ANC) genes and actin (ACT1) or actin-binding protein (SAC6, ABP1, TPM1) genes. The anc mutations were found to exhibit allele-specific noncomplementing interactions with different act1 mutations. In addition, mutant alleles of four ANC genes (ANC1, ANC2, ANC3 and ANC4) were tested for interactions with null alleles of actin-binding protein genes. An anc1 mutant allele failed to complement null alleles of the SAC6 and TPM1 genes that encode yeast fimbrin and tropomyosin, respectively. Also, synthetic lethality between anc3 and sac6 mutations, and between anc4 and tpm1 mutations was observed. Taken together, the above results strongly suggest that the ANC gene products contribute to diverse aspects of actin function. Finally, we report the results of tests of two models previously proposed to explain extragenic noncomplementation.