Characterization of phenolic constituents and evaluation of antioxidant properties of leaves and stems of Eriocephalus africanus
Characterization of phenolic constituents and evaluation of antioxidant properties of leaves and stems of Eriocephalus africanus
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DOI：
10.1016/j.arabjc.2015.04.018
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发表时间：
2018-01-01
影响因子：
6
通讯作者：
Cardoso, Susana M.
中科院分区：
文献类型：
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作者：
Catarino, Marcelo D.;Silva, Artur M. S.;Cardoso, Susana M.
非洲角头草是一种南非特有的菊科植物，在地中海地区被移植，通常用于烹饪和传统医学。尽管民间医学声称这种植物对健康有益，但仍然缺乏科学数据来支持这一信息。本研究描述了非洲藤茎叶氢乙醇提取物的主要酚类成分，并对其抗氧化性能进行了评价。采用LC-DAD-ESI/MS对非洲藤茎叶中的酚类成分进行了鉴定，并通过核磁共振分析进一步证实了两种主要hplc洗脱化合物的结构。两种提取物均含有丰富的单咖啡酰奎宁酸和二咖啡酰奎宁酸，分别占茎和叶提取物总定量酚类物质的约90%和74%。此外，在叶提取物中发现了相当数量的碘二醇己脲。必须强调的是，这些化合物与其他次要的酚酸（即其他咖啡酸，阿魏酸和原儿茶酸衍生物）和类黄酮（包括橙皮苷和环二醇）在这里是首次被鉴定出来。此外，通过体外2,2-二苯基-1-苦味酰肼（DPPH.）自由基清除能力、铁还原抗氧化能力和脂质过氧化抑制能力三种方法测定了提取物的抗氧化能力，揭示了其良好的性能。总的来说，这些结果对于阐明非洲藤的酚类成分以及了解该药用植物的生物学特性具有重要的贡献。(C) 2015年作者。制作和托管由爱思唯尔B.V.
Eriocephalus africanus, an Asteraceae plant endemic from South Africa and naturalized in the Mediterranean region, is commonly used in culinary and in traditional medicine. Despite the claimed health benefits attributed to this plant by the folk medicine, there is still a lack of scientific data to support this information.The present study describes the main phenolic composition of hydroethanolic extracts from stems and leaves of E. africanus, along with the assessment of their antioxidant properties. The identification of the phenolic constituents on the stems and leaves from E. africanus was carried out by LC-DAD-ESI/MS n, and the structures of the two major HPLC-eluted compounds were further confirmed by NMR analysis.Both extracts were rich in mono-and di-caffeoylquinic acids, which accounted for approximately 90% and 74% of total quantified phenolics, for the stems and leaves extracts, respectively. Besides, eriodictyol-hexuronide was found in considerable amounts in the extract from the leaves. One must highlight that these compounds together with other minor phenolic acids (namely other caffeoyl, ferulic and protocatechuic acid derivatives) and flavonoids (including hesperetin and eriodictyol) were herein identified for the first time.Furthermore, through three in vitro methods, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH.) radical scavenging, ferric reducing antioxidant power and lipid peroxidation inhibition capacity assays, the antioxidant capacity of the extracts was measured, revealing promising properties.Overall, these results are an important contribution for the elucidation of the phenolic composition of E. africanus, as well as for the understanding of the biological properties of this medicinal plant species. (C) 2015 The Authors. Production and hosting by Elsevier B.V.