Understanding Cancer Drug Resistance by Developing and Studying Resistant Cell Line Models
Understanding Cancer Drug Resistance by Developing and Studying Resistant Cell Line Models
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DOI：
10.2174/1568009616666151113120705
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发表时间：
2016-01-01
影响因子：
3
通讯作者：
Helena Vasconcelos, M.
中科院分区：
文献类型：
--
作者：
Xavier, Cristina P. R.;Pesic, Milica;Helena Vasconcelos, M.
尽管目前临床上有大量的抗癌药物，但由于耐药性而导致的治疗失败非常频繁。有必要查明对不同药物产生耐药性的机制，以确定预防和避免这种耐药性的方法。事实上，识别新的治疗靶点以克服癌症耐药性仍然是药物发现和开发中的主要挑战之一。用于鉴定耐药机制和新的治疗靶点的方法在很大程度上取决于癌症耐药细胞系的建立。建立这样的耐药细胞系是费力和耗时的，并且已经描述了各种不同的方法。本文综述了用于建立癌症耐药细胞系和鉴定其耐药机制的方法。此外，这篇综述强调了在癌细胞中发现的最常见的耐药机制。
Despite the enormous number of anticancer drugs presently available in the clinic, treatment failure due to drug resistance is very frequent. The identification of mechanisms of resistance to different drugs is necessary, in order to identify ways to prevent and circumvent such resistance. Indeed, the identification of novel therapeutic targets to overcome cancer drug resistance remains one of the major challenges in drug discovery and development. The methods employed to identify drug resistance mechanisms and novel therapeutic targets depend greatly on the establishment of cancer drug resistant cell lines. The establishment of such drug resistant cell lines is laborious and time-consuming and various different approaches have been described. This manuscript reviews the methodologies that have been used to create cancer drug resistant cell lines and to identify their mechanisms of resistance. In addition, this review highlights the most frequent drug resistance mechanisms found in cancer cells.